91猫先生

简昆鎰

职称：副教授

研究室名称：蛋白质体分析实验室

最高学歷：Ph.D.

学校/国家：清华大学/台湾

分机号码：5971

电子邮件帐号： kychien@mail.cgu.edu.tw

个人网页网址：

研究室现有： 博士后研究员? 0 人

博士班研究生? 2? 人

硕士班研究生 0 人

专任研究助理? 0? 人

大学部专题生? 0?? 人

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研究方向及研究室特色：

蛋白质体学研究是指全面性的探讨蛋白质表现程度的学门。在生物医学领域中，通常着重於找出两个样品之间相对差异表现的蛋白。由於分析技术的快速进步，使得蛋白质体分析的覆盖率，已逐渐赶上基因体学研究。其中最主要的两项技术為质谱学及胜肽分离技术。本实验室主要的研究兴趣就是针对这两项技术进行研发及改进，以提升蛋白质体数据的广泛度、灵敏度、可信度及自动化程度。
对於胜肽分离系统的改进，我们建立了多种奈米级多维液相分离系统，不论是一般性的整体蛋白体分析，或是特定族群胜肽的纯化分离，其效能均已达国际一流的水準。利用这些系统，我们可以从30ug的细胞蛋白萃取物中鑑定出6000-9000个蛋白，甚至可从200微克蛋白中鑑定出近万条磷酸化胜肽。至於在质谱测量方面，我们着重於开发崭新的同位素标定技术，以提高质谱定量数据的可靠性。目前我们已建立两种标定技术，其中之ㄧ可进行整体蛋白体定量分析，另一则专注的定量特定胺基酸的氧化程度。这些技术都将运用於疾病蛋白体学研究，尤其将着重於分析临床的组织或体液样品。

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最近五年所发表论文：

1. Tang WF, Huang RT, Chien KY, Huang JY, Lau KS, Jheng JR, Chiu CH, Wu TY, Chen CY, Horng JT. Host MicroRNA miR-197 Plays a Negative Regulatory Role in the Enterovirus 71 Infectious Cycle by Targeting the RAN Protein. J Virol. 2015 Nov 18;90(3):1424-38.

2. Geng J, Sun X, Wang P, Zhang S, Wang X, Wu H, Hong L, Xie C, Li X, Zhao H, Liu Q, Jiang M, Chen Q, Zhang J, Li Y, Song S, Wang HR, Zhou R, Johnson RL, Chien KY, Lin SC, Han J, Avruch J, Chen L, Zhou D. Kinases Mst1 and Mst2 positively regulate phagocytic induction of reactive oxygen species and bactericidal activity. Nat Immunol. 2015 Nov;16(11):1142-52.

3. Huang HW, Liu BS, Chien KY, Chiang LC, Huang SY, Sung WC, Wu WG. Cobra venom proteome and glycome determined from individual snakes of Naja atra reveal medically important dynamic range and systematic geographic variation. J Proteomics. 2015 Oct 14;128:92-104.

4. Chang KP, Lin SJ, Liu SC, Yi JS, Chien KY, Chi LM, Kao HK, Liang Y, Lin YT, Chang YS, Yu JS. Low-molecular-mass secretome profiling identifies HMGA2 and MIF as prognostic biomarkers for oral cavity squamous cell carcinoma. Sci Rep. 2015 Jul 3;5:11689.

5. Gopinath RK, You ST, Chien KY, Swamy KB, Yu JS, Schuyler SC, Leu JY. The Hsp90-dependent proteome is conserved and enriched for hub proteins with high levels of protein-protein connectivity. Genome Biol Evol. 2014 Oct 13;6(10):2851-65.

6. Chen CD, Wang CL, Yu CJ, Chien KY, Chen YT, Chen MC, Chang YS, Wu CC, Yu JS. Targeted proteomics pipeline reveals potential biomarkers for the diagnosis of metastatic lung cancer in pleural effusion. J Proteome Res. 2014 Jun 6;13(6):2818-29.

7. Lin SJ, Chang KP, Hsu CW, Chi LM, Chien KY, Liang Y, Tsai MH, Lin YT, Yu JS. Low-molecular-mass secretome profiling identifies C-C motif chemokine 5 as a potential plasma biomarker and therapeutic target for nasopharyngeal carcinoma. J Proteomics. 2013 Dec 6;94:186-201.

8. Chang KP, Wang CL, Kao HK, Liang Y, Liu SC, Huang LL, Hseuh C, Hsieh YJ, Chien KY, Chang YS, Yu JS, Chi LM.Overexpression of caldesmon is associated with lymph node metastasis and poorer prognosis in patients with oral cavity squamous cell carcinoma. Cancer. 2013 Nov 15;119(22):4003-11.

9. Chen JT, Ho CW, Chi LM, Chien KY, Hsieh YJ, Lin SJ, Yu JS. Identification of the lamin A/C phosphoepitope recognized by the antibody P-STM in mitotic HeLa S3 cells. BMC Biochem. 2013 Jul 19;14:18.

10. Chen CL, Lin TS, Tsai CH, Wu CC, Chung T, Chien KY, Wu M, Chang YS, Yu JS, Chen YT. Identification of potential bladder cancer markers in urine by abundant-protein depletion coupled with quantitative proteomics. J Proteomics. 2013 Jun 24;85:28-43.

11. Dong YM, Chien KY, Chen JT, Lin SJ, Wang TC, Yu JS. Site-specific separation and detection of phosphopeptide isomers with pH-mediated stacking capillary electrophoresis-electrospray ionization-tandem mass spectrometry. J Sep Sci. 2013 May;36(9-10):1582-9.

12. Chen CL, Lai YF, Tang P, Chien KY, Yu JS, Tsai CH, Chen HW, Wu CC, Chung T, Hsu CW, Chen CD, Chang YS, Chang PL, Chen YT. Comparative and targeted proteomic analyses of urinary microparticles from bladder cancer and hernia patients. J Proteome Res. 2012 Dec 7;11(12):5611-29.

13. Wang CI, Chien KY, Wang CL, Liu HP, Cheng CC, Chang YS, Yu JS, Yu CJ. Quantitative proteomics reveals regulation of karyopherin subunit alpha-2 (KPNA2) and its potential novel cargo proteins in nonsmall cell lung cancer. Mol Cell Proteomics. 2012 Nov;11(11):1105-22.

14. Chen CY, Weng YH, Chien KY, Lin KJ, Yeh TH, Cheng YP, Lu CS, Wang HL. (G2019S) LRRK2 activates MKK4-JNK pathway and causes degeneration of SN dopaminergic neurons in a transgenic mouse model of PD. Cell Death Differ. 2012 Oct;19(10):1623-33.

15. Lin SY, Li TY, Liu Q, Zhang C, Li X, Chen Y, Zhang SM, Lian G, Liu Q, Ruan K, Wang Z, Zhang CS, Chien KY, Wu J, Li Q, Han J, Lin SC. Protein phosphorylation-acetylation cascade connects growth factor deprivation to autophagy. Autophagy. 2012 Sep;8(9):1385-6.

16. Hsieh YJ, Chien KY, Lin SY, Sabu S, Hsu RM, Chi LM, Lyu PC, Yu JS. Photofrin binds to procaspase-3 and mediates photodynamic treatment-triggered methionine oxidation and inactivation of procaspase-3. Cell Death Dis. 2012 Jul 12;3:e347.

17. Sun NK, Huang SL, Chien KY, Chao CC. Golgi-SNARE GS28 potentiates cisplatin-induced apoptosis by forming GS28-MDM2-p53 complexes and by preventing the ubiquitination and degradation of p53. Biochem J. 2012 Jun 1;444(2):303-14

18. Lin SY, Li TY, Liu Q, Zhang C, Li X, Chen Y, Zhang SM, Lian G, Liu Q, Ruan K, Wang Z, Zhang CS, Chien KY, Wu J, Li Q, Han J, Lin SC. GSK3-TIP60-ULK1 signaling pathway links growth factor deprivation to autophagy. Science. 2012 Apr 27;336(6080):477-81.

19. Feng Y, Chien KY, Chen HL, Chiu CH. Pseudogene recoding revealed from proteomic analysis of salmonella serovars. J Proteome Res. 2012 Mar 2;11(3):1715-9.

20. Tsai MH, Wu CC, Peng PH, Liang Y, Hsiao YC, Chien KY, Chen JT, Lin SJ, Tang RP, Hsieh LL, Yu JS. Identification of secretory gelsolin as a plasma biomarker associated with distant organ metastasis of colorectal cancer. J Mol Med (Berl).? 2012 Feb;90(2):187-200.

21. Wu ZZ, Sun NK, Chien KY, Chao CC. Silencing of the SNARE protein NAPA sensitizes cancer cells to cisplatin by inducing ERK1/2 signaling, synoviolin ubiquitination and p53 accumulation. Biochem Pharmacol. 2011 Dec 1;82(11):1630-40.

22. Chang KP, Yu JS, Chien KY, Lee CW, Liang Y, Liao CT, Yen TC, Lee LY, Huang LL, Liu SC, Chang YS, Chi LM. Identification of PRDX4 and P4HA2 as metastasis-associated proteins in oral cavity squamous cell carcinoma by comparative tissue proteomics of microdissected specimens using iTRAQ technology. J Proteome Res. 2011 Nov 4;10(11):4935-47.

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