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生化暨分子生物学科

生化暨分子生物学科

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刘轩

刘轩

职称：教授

研究室名称：癌症功能基因体学实验室

最高学歷：博士.

学校/国家：长庚大学/台湾

分机号码：3526

电子邮件帐号：liu-hsuan@mail.cgu.edu.tw

个人网页网址：https://cgmmrc.cgu.edu.tw/p/406-1029-69430,r1661.php?Lang=zh-tw

研究室现有：博士后研究员1人

博士班研究生1人

硕士班研究生3人

专任研究助理5人

大学部专题生3人

Update：2021/09/13

研究方向及研究室特色：

高通量(high-throughput)生物实验技术揭开后基因体世代 (post-genomic era)的序幕，而近年呈现爆炸性发展的次世代定序生物技术，更进一步将生物实验技术推向高效率、高精度、高準确性之境界，為生命科学研究领域创造极佳的契机。次世代定序方法目前也已被广泛应用於各类型的生命科学研究工作上，研究人员可针对DNA与RNA进行高速定序，搭配后端生物资讯 (bioinformatics)的应用，进行基因序列变异分析、基因结构异常分析、基因表现量分析、微小RNA (small RNA)表现量分析、基因体甲基化修饰等之研究。

本实验室的研究主要在建立和应用各种NGS技术平台於癌症基因体及转录体上的研究，搭配生物资讯的分析, 系统性的找出癌症重要的“生物特徵”及相关病理特徵，利用大數據庫運算，建構其可能參与之調控網絡，并利用细胞和动物模式进行深入的分子机制探讨及验証。近年主要研究包括：

  1. 建立台湾本土癌症组织之功能性基因體體學數據庫

建立完整的台湾本土癌症组织(口腔癌/食道癌/大肠直肠癌/脑癌)之功能性基因体体学数据库，并利用生物资讯方式，结合国外大数据库，系統性的分析找出与臨床的早期診斷、疾病的治療与用藥、抗藥性、存活率与再復性相关的重要分子，完整的描繪這些變體的病理作用機制以及調控網絡。針對其中重要的變異基因，進一步使用細胞和動物模式研究來深入探討及驗証這些癌症相关標的之分子機制。

  1. 开发分析非编码RNA调控网络之生物资讯工具

利用非编码RNA已知之生物特性 (long noncoding RNA/circRNA/miRNA)，结合大数据库，开发分析非编码RNA调控网路之生物资讯分析平台。进一步结合细胞和动物模式，验証其中新颖之非编码RNA的调控分子机制。

  1. 开发次世代定序法於癌症液态检体之应用

外泌體組成不僅反應了起源細胞，且富含与細胞轉化相关的生物分子，因此已成為近年來液體活檢方法中最有潛力的目標。實驗室建構了一套只需1 cc的血浆，即可快速提取外泌核糖核酸方法，利用次世代定序法验証了此方法明显优化并提升样本製备的稳定性。我们更进一步全面性的建立了健康人及多种癌症之外泌微核糖核酸体学数据库 (口腔癌、食道癌、肺癌、大肠直肠癌)，结合临床资讯，系统性的寻找具早检测、长期监测治疗反应以及预后之多面向的生物标誌。因应精準医学方面之应用，更进一步开发一套快速检测晶片系统，可精準且多面向的监测癌症及治疗进程。利用多种生物资讯工具，完整描绘出外泌微核糖核酸在癌症进程所扮演的功能角色，并在细胞及动物模式上进行深入的机制研究。

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最近五年所发表论文：

  1. Chiang S-F, Huang HH, Tsai W-S, Chin-Ming Tan B, Yang C-Y, Huang P-J, Yi-Feng Chang I, Lin J, Lu P-S, Chin E, Liu Y-H, Yu J-S, Chiang J-M, Hung H-Y, Liu H* (2021, Mar). Comprehensive functional genomic analyses link APC somatic mutation and mRNA-miRNA networks to the clinical outcome of stage-III colorectal cancer patients. Biomedical Journal, Available online 16 March 2021. MOST 106-2320-B-182-035-MY3. 本人為通讯作者. (SCI)
  2. Min-Hua Tseng, Wen-Lang Fan, Hsuan Liu, Chia-Yu Yang, Jhao-Jhuang Ding , Hwei-Jen Lee , Shih-Ming Huang, Shih-Hua Lin and Jing-Long Huang (2021, Feb). Complement Factor I Mutation May Contribute to Development of Thrombotic Microangiopathy in Lupus Nephritis. Frontiers in Medicine, Feb 5;7:621609. (SCI)
  3. Wen-Sy Tsai, Wei-Shan Hung, Tzu-Min Wang, Hsuan Liu, Chia-Yu Yang, Shao-Min Wu, Hsueh-Ling Hsu, Yu-Chiao Hsiao, Hui-Ju Tsai, Ching-Ping Tseng (2020, Oct). Circulating tumor cell enumeration for improved screening and disease detection of patients with colorectal cancer. Biomedical Journal. MOST 106-2320-B-182-027-MY3. (SCI)
  4. Chen YT, Chang IY, Kan CH, Liu YH, Kuo YP, Tseng HH, Chen HC, Liu H, Chang YS, Yu JS, Chang KP, Tan BC. (2020, Aug). circRNAome Profiling in Oral Carcinoma Unveils a Novel circFLNB that Mediates Tumour Growth-Regulating Transcriptional Response. Cells, 10;9(8):1868. MOST 106-2320-B-182-035-MY3. (SCI)
  5. Yang CY, Liu CR, Chang IY, OuYang CN, Hsieh CH, Huang YL, Wang CI, Jan FW, Wang WL, Tsai TL, Liu H, Tseng CP, Chang YS, Wu CC, Chang KP (2020, Jun). Cotargeting CHK1 and PI3K Synergistically Suppresses Tumor Growth of Oral Cavity Squamous Cell Carcinoma in Patient-Derived Xenografts. Cancers (Basel), 29;12(7):1726.. MOST 107-2314-B-182-075-MY3. (SCI)
  6. Wu SM, Tsai WS, Chiang SF, Lai YH, Ma CP, Wang JH, Lin J, Lu PS5, Yang CY, Tan BC, Liu H* (2020, Mar). Comprehensive transcriptome profiling of Taiwanese colorectal cancer implicates an ethnic basis for pathogenesis. Sci Rep, 10(1):4526. MOST 106-2320-B-182-035-MY3.本人為通讯作者. (SCI)
  7. Chung AK, OuYang CN, Liu H, Chao M, Luo JD, Lee CY, Lu YJ, Chung IC, Chen LC, Wu SM, Tsang NM, Chang KP, Hsu CL, Li HP, Chang YS. (2019, Sep). Targeted sequencing of cancer-related genes in nasopharyngeal carcinoma identifies mutations in the TGF-β pathway. Cancer Medicine, 8(11):5116-5127. MOST 105-2320-B-182-034-MY3. (SCI)
  8. Kuo YP, Ma CP, Chen HW, Chen YT, Lai YH, Liu H, Kuo RL, Chin-Ming Tan B. (2019, Sep). A novel antisense RNA ASPACT confers multi-level suppression of PACT and associated signalling. RNA Biology, 16(9):1263-1274. (SCI).
  9. Ma CP, Liu H, Yi-Feng Chang, Wang WC, Chen YT, Wu SM, Chen HW, Kuo YP, Shih CT, Li CY, Tan BC. (2019, May). ADAR1 promotes robust hypoxia signaling via distinct regulation of multiple HIF-1α-inhibiting factors.EMBO Report, 20(5): pii: e47107. MOST 106-2320-B-182-042-MY3. (SCI)
  10. Lai CY*, Liu H*, Tin KX, Huang Y, Yeh KH, Peng HW, Chen HD, He JY, Chiang YJ, Liu CS, Weng SY, Tao MH, Yen JJ, Yang-Yen HF. (2019, Jan). Identification of UAP1L1 as a critical factor for protein O-GlcNAcylation and cell proliferation in human hepatoma cells. . Oncogene, 38(3):317-331.. MOST 102-2320-B-001-024-MY3. 本人為第一作者. (SCI)
  11. You GR, Cheng AJ, Lee LY, Huang YC, Liu H, Chen YJ, Chang JT. (2019, Jan). Prognostic signature associated with radioresistance in head and neck cancer via transcriptomic and bioinformatic analyses. BMC Cancer, 19(1):64. (SCI).
  12. Huang YW, Chiang MF, Ho CS, Hung PL, Hsu MH, Lee TH, Chu JL, Liu H, Tang P, Ng VW, Lin DS (2018, Dec). A Transcriptome Study of Progeroid Neurocutaneous Syndrome Reveals POSTN As a New Element in Proline Metabolic Disorder. Aging and Disease, 9(6):1043-1057. (SCI).
  13. Chao A, Lin CY, Wu RC, Lee YS, Lee LY, Tsai CL, Yang LY, Liu H, Chen SJ, Wang TH, Lai CH (2018, Nov). The combination of everolimus and terameprocol exerts synergistic antiproliferative effects in endometrial cancer: molecular role of insulin-like growth factor binding protein 2. J Mol Med (Berl), 96(11):1251-1266. MOST 105-2320-B-182A-005. (SCI).
  14. Chang S, Bok P, Tsai CY, Sun CP, Liu H, Deussing JM, Huang GJ (2018, Aug). NPTX2 is a key component in the regulation of anxiety. Neuropsychopharmacology, 43(9):1943-1953. (SCI).
  15. Chen YT, Chang IY, Liu H, Ma CP, Kuo YP, Shih CT, Shih YH, Kang L, Tan BC (2018, Jun). Tumor-associated intronic editing of HNRPLL generates a novel splicing variant linked to cell proliferation. J Biol Chem, 293(26) 10158–10171. MOST 105-2320-B-182-017. (SCI).
  16. Yang CY, Yeh YM, Yu HY, Chin CY, Hsu CW, Liu H, Huang PJ, Hu SN, Liao CT, Chang KP, Chang YL. (2018, May). Oral Microbiota Community Dynamics Associated With Oral Squamous Cell Carcinoma Staging. Front Microbiol, 9:862. (SCI).
  17. Tsai KW, Lo YH, Liu H, Yeh CY, Chen YZ, Hsu CW, Chen WS, Wang JH (2018, Mar). Linc00659, a long noncoding RNA, acts as novel oncogene in regulating cancer cell growth in colorectal cancer. Molecular Cancer, 17(1):72. (SCI).
  18. Lai YH, Liu H, Chiang WF, Chen TW, Chu LJ, Yu JS, Chen SJ, Chen HC, Tan BC (2018, Jan). MiR-31-5p-ACOX1 Axis Enhances Tumorigenic Fitness in Oral Squamous Cell Carcinoma Via the Promigratory Prostaglandin E2. Theranostics, 8(2):486-504. MOST 106-2320-B-182-035-MY3. (SCI).
  19. Wu SM*, Liu H*, Huang PJ, Chang IY, Lee CC, Yang CY, Tsai WS, Tan BC. (2018, Jan). circlncRNAnet: An integrated web-based resource for mapping functional networks of long or circular forms of non-coding RNAs. , 7 (1):1-10. MOST 106-2320-B-182-035-MY3. 本人為第一作者(SCI).
  20. Chen TW*, Lee CC*, Liu H*, Wu CS*, Pickering CR*, Huang PJ, Wang J, Chang YF, Yeh YM, ChenCD, Li HP, Luo JD, Tan BC, Chan EH, Hsueh C, Chu LJ, Chen YT, Zhang B, Yang CY, Wu CC, Hsu CW, See LC, Tang P, Yu JS, Liao WC, Chiang KW, Rodriguez H, Myers JN., Chang KP, Chang YS (2017, Sep). APOBEC3A is an oral cancer prognostic biomarker in Taiwanese carriers of an APOBEC deletion polymorphism. Nature Communication, 8 (1):465.. MOST 103-2632-B-182-001. 本人為第一作者. (SCI)
  21. Yang CC, Chen YT, Chang YF, Liu H, Kuo YP, Shih CT, Liao WC, Chen HW, Tsai WS, Tan, BC (2017, May). ADAR1 mediated 3’ UTR editing and expression control of anti-apoptosis genes fine-tunes cellular apoptosis response. Cell Death and Disease, 8(5):e2833. MOST 105-2320-B-182-017. (SCI).
  22. Chen H, Hsiao YC, Chiang SF, Wu CC, Lin YT, Liu H, Zhao H, Chen JS, Chang YS, Yu JS. (2016, Aug). Quantitative analysis of wild-type and V600E mutant BRAF proteins in colorectal carcinoma using immunoenrichment and targeted mass spectrometry. Analytica Chimica Acta, 933:144-55. (SCI).

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