91猫先生

研究方向及研究室特色(Lab & Research Interest)

1.       探讨低氧预处理之干细胞对Bleomycin诱导肺纤维化的小鼠之治疗效果    

特发性肺纤维化 (IPF) 目前被认為是一种原因不明之呼吸道弥漫性发炎性疾病，一连串发炎反应及间质细胞过度累积為造成肺部细胞功能异常之主要原因。研究发现当肺损伤所诱发之发炎反应，其炎性细胞所释放之细胞激素会刺激大量产生，激活罢骋贵-β1之讯号途径及诱导胶原蛋白累积而导致肺纤维化。近来许多研究指出，利用肝细胞生长因子 (HGF) 之基因治疗法有明显改善经由Bleomycin所诱导之细胞凋亡、发炎反应及肺纤维化等病症。另外，目前利用间叶干细胞 (MSC)治疗肺损伤动物模式已有显着成效，MSC除了降低伤害外亦针对损伤部位进行修復；但近来研究亦指出，移植之MSC容易受到损伤微环境之伤害而死亡，而造成应用MSC治疗的障碍。再者，科学家利用预先给予低氧预处理(preconditioning)之干细胞治疗心肌梗塞等疾病时，发现其成功改善移植后之干细胞的存活率及增加治疗疾病的效果。因此本研究拟探讨低氧预处理之干细胞在Bleomycin诱导肺纤维化的小鼠之疗效。

2.       探讨多重抗药性癌细胞抗药性的分子机制
      多重抗药性癌细胞目前已成為临床上以化疗药物治疗恶性肿瘤的主要障碍。此种情形主要由於癌细胞细胞表面药物通透性的改变而造成。儘管多重抗药性抑制剂已被发现和研究多年，但始终无法有效应用於临床上治疗，寻找新颖的调控途径来杀死多重抗药性癌细胞成為当务之急。近年科学家发现一个以蛋白酶体抑制的新颖方法可以诱发癌细胞走向细胞凋亡。儘管许多研究已证实蛋白酶体抑制剂的疗效。不过，仍有一部分的病人对於蛋白酶体抑制剂的治疗失效。因此，我们假设这些病人体内的多重抗药性癌细胞可能衍生出新颖机制来拮抗蛋白酶体抑制剂bortezomib的毒杀效果。

论文与着作(笔耻产濒颈肠补迟颈辞苍)

Selected Publications (2018-2024)

Tung YT, Chen HL, Wu HS, Ho MH, Chong KY, Chen CM. Kefir Peptides Prevent Hyperlipidemia and Obesity in High-Fat-Diet-Induced Obese Rats via Lipid Metabolism Modulation.  Mol Nutr Food Res. 62(3). doi: 10.1002/mnfr.201700505, 2018

Chang YW, Tseng CP, Lee CH, Hwang TL, Chen YL, Su MT, Chong KY , Lan YW, Wu CC, Chen KJ, Lu FH, Liao HR, Hsueh C, Hsieh PW.  β-Nitrostyrene derivatives attenuate LPS-mediated acute lung injury via the inhibition of neutrophil-platelet interactions and NET release. Am J Physiol Lung Cell Mol Physiol. 314: L654-69, 2018.

Huang TT, Lan YW, Ko YF, Chen CM, Lai HC, Ojcius DM, Martel J, Young JD, Chong KY*. Antrodia cinnamomea produces anti-angiogenic effects by inhibiting the VEGFR2 signaling pathway. J Ethnopharmacol. 220: 239-49, 2018.

Hiew MSY, Cheng HP, Huang CJ, Chong KY, Cheong SK, Choo KB, Kamarul T. Incomplete cellular reprogramming of colorectal cancer cells elicits an epithelial/ mesenchymal hybrid phenotype. J Biomed Sci. 25: 57, 2018.

Lee IT, Lin CF, Huang YL, Chong KY, Hsieh MF, Huang TH, Cheng CY. Protective mechanisms of resveratrol derivatives against TNF-α-induced inflammatory responses in rat mesangial cells. Cytokine. 113: 380-92, 2018.

Huang TT, Lan YW, Chen CM, Ko YF, Ojcius DM, Martel J, Young JD, Chong KY*.  Antrodia cinnamomea induces anti-tumor activity by inhibiting the STAT3 signaling pathway in lung cancer cells.  Sci Rep. 9: 5145, 2019.

Lan YW, Yang JC, Yen CC, Huang TT, Chen YC, Chen HL, Chong KY*, Chen CM*. Predifferentiated amniotic fluid mesenchymal stem cells enhance lung alveolar epithelium regeneration and reverse elastase-induced pulmonary emphysema. Stem Cell Res Ther. 10: 163, 2019.

Chen HL, Hung KF, Yen CC, Laio CH, Wang JL, Lan YW, Chong KY, Fan HC, Chen, CM.  Kefir peptides alleviate particulate matter <4 μm (PM4.0)-induced pulmonary inflammation by inhibiting the NF-κB pathway using luciferase transgenic mice. Sci Rep 9: 11529, 2019.

Yen CC, Chang WH, Tung MC, Chen HL, Liu HC, Liao CH, Lan YW, Chong KY, Yang SH, Chen CM. Lactoferrin Protects Hyperoxia-Induced Lung and Kidney Systemic Inflammation in an In Vivo Imaging Model of NF-κB/Luciferase Transgenic Mice. Mol Imaging Biol. 22: 526-38, 2020.

Ou-Yang H, Wu SC, Sung LY, Yang SH, Yang SH, Chong KY, Chen CM. Article stat3 is an upstream regulator of granzyme g in the maternal-to-zygotic transition of mouse embryos. Int J Mol Sci. 22: 460, 2021

Lin SS, Niu CC, Yuan LJ, Tsai TT, Lai PL, Chong KY, Wei KC, Huang CY, Lu ML, Yang CY, Ueng SWN. Mir-573 regulates cell proliferation and apoptosis by targeting Bax in human degenerative disc cells following hyperbaric oxygen treatment. J Orthop Surg Res. 16: 16, 2021.

Tu MY, Han KY, Lan YW, Chang KY , Lai CW, Staniczek T, Lai CY, Chong KY*, Chen CM. Association of 罢骋贵-β1 and IL-10 Gene Polymorphisms with Osteoporosis in a Study of Taiwanese Osteoporotic Patients. Genes (Basel). 12(6): 930, 2021. 

Huang CH# , Chong KY# , Lei KF. Analysis of the Internal Hypoxic Environment in Solid Tumor Tissue Using a Folding Paper System. ACS Appl Mater Interfaces. 13(29):33885-33893, 2021

Wang JL, Tsai YT, Lin CH, Cidem A, Staniczek T, Chang GRL, Yen CC, Chen W, Chong KY*, Chen CM.  Benefits of metformin combined with pemetrexed-based platinum doublets as a first-line therapy for advanced lung adenocarcinoma patients with diabetes. Biomolecules.11(8):1252, 2021. 

Chen CF, Li HP, Chao YH, Tu MY, Yen CC, Lan YW, Yang SH, Chong KY*, Lin CC*, Chen CM*. Suppression of Dendritic Cell Maturation by Kefir Peptides Alleviates Collagen-Induced Arthritis in Mice. Front. Pharmacol. 12: 721594, 2021.

Chen YC, Lan YW, Huang SM, Yen CC, Chen W, Wu WJ, Theresa Staniczek, Chong KY, Chen CM.  Human amniotic fluid mesenchymal stem cells attenuate pancreatic cancer cell proliferation and tumor growth in an orthotopic xenograft mouse model.  Stem Cell Res Ther. 13:235, 2022.  

Liu YH, Chen YH, Ko CH, Kuo CW, Yen CC, Chen Wei, Chong KY*, and Chen CM*.  SOD3 and IL-18 Predict the First Kidney Disease-Related Hospitalization or Death during the One-Year Follow-Up Period in Patients with End-Stage Renal Disease. Antioxidants. 11, 1198. 2022.

Wen YH, Lee CF, Chen YJ, Chang GJ* and Chong KY*.  Risks in Induction of Platelet Aggregation and Enhanced Blood Clot Formation in Platelet Lysate Therapy: A Pilot Study.  J Clin Med. 11: 3972, 2022. 

Huang TT, Chen CM, Chen LG, Lan YW, Huang TH, Choo KB and Chong KY*.  2,3,5,4'-tetrahydroxystilbene -2-O-β-D-glucoside ameliorates bleomycin-induced pulmonary fibrosis via regulating pro-fibrotic signaling pathways.  Front. Pharmacol. 13: 997100, 2022.

Huang TT, Chen CM, Lan YW, Lin SS, Choo KB, Chong KY*. Blockade of c-Met-Mediated Signaling Pathways by E7050 Suppresses Growth and Promotes Apoptosis in Multidrug-Resistant Human Uterine Sarcoma Cells. Int J Mol Sci. 23: 14884, 2022.

Huang TT, Chen CM, Lin SS, Lan YW, Cheng HC, Choo KB, Wang CC, Huang TH, Chong KY. E7050 Suppresses the Growth of Multidrug-Resistant Human Uterine Sarcoma by Inhibiting Angiogenesis via Targeting of VEGFR2-Mediated Signaling Pathways. Int J Mol Sci.24(11): 9606, 2023.

Lin SS, Ueng SWN, Chong KY, Chan YS, Tsai TT, Yuan LJ, Liu SJ, Yang CY, Hsiao HY, Hsueh YJ, Chen CA, Niu CC. Effects of Hyperbaric Oxygen Intervention on the Degenerated Intervertebral Disc: From Molecular Mechanisms to Animal Models. Cells. 12(16): 2111, 2023.

Lan YW, Chen WR, Chang GR, Chen YC, Chong KY, Chuang KC, Kao YT, Chen MS, Chen CM. Aldo-keto reductase family 1 member A1 (AKR1A1) exerts a protective function in alcohol-associated liver disease by reducing 4-HNE accumulation and p53 activation. Cell Biosci. 14(1):18, 2024.

Yen CC, Wu PY, Ou-Yang H, Chen HL, Chong KY, Chang RL, Chen CM. Production of Bioactive Porcine Lactoferrin through a Novel Glucose-Inducible Expression

System in Pichia pastoris: Unveiling Antimicrobial and Anticancer

Functionalities. Int J Mol Sci. 25(3):1818, 2024.

Lan YW, Chen CE, Huang TT, Huang TH, Chen CM, Chong KY*. Antrodia cinnamomea extract alleviates bleomycin-induced pulmonary fibrosis in mice by inhibiting the mTOR pathway. Biomed J. 2024 , 100720.

Book Chapter

Chan AWS, Chong KY and Schatten GP. Transgenic bovine embryo selection using green fluorescent protein. In: Hicks BW (ed) Green Fluorescent Protein: Method in Molecular biology 183. Humana Press, New Jersey, 2002, pp 201-214

Chan AWS, Chong KY and Schatten GP. Production of Transgenic Nonhuman Primates. In Pinkert CA (ed) Transgenic Animal Technology, 2nd edn. Academic Press, San Diego, 2002. pp 359-394.4.    

Lan YW, Chen CM, Chong KY. In Vitro Methods to Evaluate the Effects of Mesenchymal Stem Cells on 罢骋贵-β1-Induced Pulmonary Fibrosis.  In Vitro Models for Stem Cell Therapy. 2021, pp 83-92.