91猫先生

研究方向及研究室特色(Lab & Research Interest)

1. 研究氧化压力与感染性疾病之关係

      宿主因素影响病原体的感染性及致病性，然而氧化还原状态為宿主因素之一。我们的初步研究结果显示：宿主氧化还原态若偏向氧化态时，肠病毒的复製及所引至之细胞病变效应即加剧。粒线体状态在病毒感染时呈异常。目前，我们使用分子技术、动物模式结合蛋白质体学，探讨其机转。感染模型上建立肠病毒71型口餵感染模型。另外，结合传统中医药方，发现降低宿主感染病毒时的氧化压力能进一步降低其病毒致病率。

2. 研究粒线体与氧化压力在癌症发生之角色

      粒线体被认為是活性氧分子主要来源；亦是能量产生及其它生理过程如细胞淍亡之调控者。我们的初步研究结果显示：粒线体状态与癌细胞生长密不可分。目前，我们使用分子技术及动物模式，结合转译体和代谢体学，探讨其机转。同时，我们使用RNA干扰技术，研究粒线体及氧化压力对癌症发生之角色。

3. 研究胰岛代谢讯息传递在第二型糖尿病病理角色

        胰岛β細胞能分泌胰岛素，為調節體內血糖之平衡中不可或缺的角色。β細胞仰賴代謝路徑以耦合胰岛素分泌，此即所謂代謝訊息傳遞(Metabolic signaling)，代謝異常發生時可以影響胰岛素分泌。第二型糖尿病發病過程中與β细胞功能息息相关。目前，利用分子技术及动物模式结合代谢质体学，深入探讨β細胞的代謝訊息傳遞及胰岛的代謝異常如何引發糖尿病的機轉。

实验室成员

        博士学生: 2人; 硕士学生: 2人; 大学部: 2人; 研究助理: 2人
已毕业博士: 2人; 硕士: 11人 (统计於2007-2018年)

论文与着作(笔耻产濒颈肠补迟颈辞苍)

最近五年所发表论文：

1.    Chen SG, Leu YL, Cheng ML, Ting SC, Liu CC, Wang SD, Yang CH, Hung CY, Sakurai H, Chen KH, Ho HY. Anti-enterovirus 71 activities of Melissa officinalis extract and its biologically active constituent rosmarinic acid. Scientific Reports.2017; 7(1):12264. (SCI)

2.    Ho HY, Lin YT, Lin G, Wu PR, Cheng ML. Nicotinamide nucleotide transhydrogenase (NNT) deficiency dysregulates mitochondrial retrograde signaling and impedes proliferation. Redox Biology. 2017;12:916-928. (SCI)

3.    Tang HY, Ho HY, Chiu DT, Huang CY, Cheng ML, Chen CM. Alterations of plasma concentrations of lipophilic antioxidants are associated with Guillain-Barre syndrome. Clinica Chimica Acta. 2017; 470:75-80. (SCI) 

4.    Chen SG, Cheng ML, Chen KH, Horng JT, Liu CC, Wang SM, Sakurai H, Leu YL, Wang SD, Ho HY. Antiviral activities of Schizonepeta tenuifolia Briq. against enterovirus 71 in vitro and in vivo. Scientific Reports. 2017; 7(1):935. (SCI)

5.    Tang HY, Wang CH, Ho HY, Wu PT, Hung CL, Huang CY, Wu PR, Yeh YH, Cheng ML. Lipidomics reveals accumulation of the oxidized cholesterol in erythrocytes of heart failure patients. Redox Biology.2017;14:499-508. (SCI)

6.    Cheng ML, Chi LM, Wu PR, Ho HY. Dehydroepiandrosterone-induced changes in mitochondrial proteins contribute to phenotypic alterations in hepatoma cells. Biochemical Pharmacology.2016;117:20-34. (SCI)

7.    Wu YH, Chiu DT, Lin HR, Tang HY, Cheng ML, Ho HY. Glucose-6-Phosphate Dehydrogenase Enhances Antiviral Response through Downregulation of NADPH Sensor HSCARG and Upregulation of NF-κB Signaling. Viruses. 2016;7:6689-6706. (SCI)

8.    Cai N, Chang S, Li Y, Li Q, Hu J, Liang J, Song L, Kretzschmar W, Gan X, Nicod J, Rivera M, Deng H, Du B, Li K, Sang W, Gao J, Gao S, Ha B, Ho HY, Hu C, Hu J, Hu Z, Huang G, Jiang G, Jiang T, Jin W, Li G, Li K, Li Y, Li Y, Li Y, Lin YT, Liu L, Liu T, Liu Y, Liu Y, Lu Y, Lv L, Meng H, Qian P, Sang H, Shen J, Shi J, Sun J, Tao M, Wang G, Wang G, Wang J, Wang L, Wang X, Wang X, Yang H, Yang L, Yin Y, Zhang J, Zhang K, Sun N, Zhang W, Zhang X, Zhang Z, Zhong H, Breen G, Wang J, Marchini J, Chen Y, Xu Q, Xu X, Mott R, Huang G J, Kendler K, Flint J. Molecular signatures of major depression. Current Biology.2015; 25(9):1146-1156. (SCI)

9.    Cheng ML, Wang CH, Shiao MS, Liu MH, Huang YY, Huang CY, Mao CT, Lin JF, Ho HY, Yang NI. Metabolic Disturbances Identified in Plasma Are Associated With Outcomes in Patients With Heart Failure Diagnostic and Prognostic Value of Metabolomics. Journal of The American College of Cardiology.2015; 65:1509-1520. (SCI)

10.Yang HC, Cheng ML, Hua YS, Wu YH, Lin HR, Liu HY, Ho HY, Chiu DTY. Glucose 6-phosphate dehydrogenase knockdown enhances IL-8 expression in HepG2 cells via oxidative stress and NF-κB signaling pathway. Journal of Inflammation-London.2015;12:34. (SCI)

11.Tang HY, Ho HY, Wu PW, Chen SH, Kuypers FA, Cheng ML, Chiu DTY. Inability to maintain GSH pool in G6PD-deficient red cells causes futile AMPK activation and irreversible metabolic disturbance. Antioxidants & Redox Signaling.2015; 22:744-759. (SCI)

12.Cheng ML, Weng SF, Kuo CH, Ho HY. Enterovirus 71 induces mitochondrial reactive oxygen species generation that is required for efficient replication. PLoS ONE .2014; 9 (11): e113234. (SCI)

13.Ho HY , Cheng ML, Chiu DTY. Glucose-6-phosphate dehydrogenase – beyond the realm of red cell biology. Free Radical Research. 2014; 48:1028-1048. (SCI)

14.Hsieh YT, Lin MH, Ho HY, Chen LC, Chen CC, Shu JC. Glucose-6-Phosphate Dehydrogenase (G6PD)-Deficient Epithelial Cells Are Less Tolerant to Infection by Staphylococcus aureus. PLoS One.2013; 8(11):e79566. (SCI)

15.Cheng ML, Ho HY, Lin HY, Lai YC, Chiu DTY. Effective NET Formation in Neutrophils from Individuals with G6PD Taiwan-Hakka is Associated with Enhanced NADP⁺ Biosynthesis. Free Radical Research.2013; 47:699-709. (SCI)

16.Yang HC, Chen TL, Wu YH, Cheng KP, Lin Y H, Cheng ML, Ho HY, Lo SJ, Chiu DTY. Glucose 6-phosphate dehydrogenase deficiency enhances germ cell apoptosis and causes defective embryogenesis in Caenorhabditis elegans. Cell Death & Disease.2013; 4:e616. (SCI)

17.Ho HY, Cheng ML, Shiao MS, Chiu DTY. Characterization of Global Metabolic Responses of G6PD-Deficient Hepatoma Cells to Diamide-Induced Oxidative Stress. Free Radical Biology & Medicine.2013; 54:71-84. (SCI)