顏秀娟 教授 / Hsiu-Chuan Yen
职称： 教授
现职： 长庚大学醫技系专任教师
电话： 5207
学歷： 美国肯塔基大学毒理学博士
专长领域： 自由基生物医学、粒线体生物医学、毒理学、临床生化学
个人网页：
研究方向及研究室特色(Lab & Research Interest)
- 本實驗室在台灣建立唯一以氣相層析/負離子化學游離質譜儀(gas chromatography/negative-ion chemical-ionization mass spectrometry; GC/NICI-MS)分析人和小鼠之體液及小鼠組織中F2-isoprostanes、isofurans、F4-neuroprostanes等分子之平台。這些分子為活體中之脂質過氧化之黃金標準指標，且我們將此平台用於研究氧化傷害在各式人類疾病或毒性的角色。目前已和林口長庚醫師合作而應用到此平台的研究主題有蜘蛛膜下腔出血（常見之出血性中風）、頭部外傷、老人癡呆症、除草劑巴拉刈(paraquat)中毒之免疫抑制治療、和鉛(Pb)及汞(Hg)中毒之螯合治療。
- 我們在人類細胞株或含粒線體DNA (mtDNA)突變之cybrids中，探討內生性coenzyme Q10含量和合成coenzyme Q10所需之人類PDSS或COQ基因和蛋白對氧化壓力或粒線體功能缺失所產生之反應及其調控。接著，我們建立two-dimensional blue native -polyacrylamide gel electrophoresis (2D BN-PAGE)技術來鑑定人類細胞粒線體中含PDSS和數個COQ蛋白之大分子蛋白複合體，也進一步發掘小鼠細胞和組織之粒線體中類似蛋白複合體的存在。
- 我們利用含致病性mtDNA突變及缺乏mtDNA之ρ0細胞株來評估以Oxford Nanopore長片段次世代定序技術分析人類粒線體DNA突變及拷貝數的最佳條件。
(2026/4/23更新，其他详见实验室网页)
论文与着作(笔耻产濒颈肠补迟颈辞苍)
近年所发表期刊论文(Publications in recent years; updated on 2024/7)
1. HC Yen*, YC Liu, CC Kan, HJ Wei, SH Lee, YH Wei, YH Feng, CW Chen, CC Huang. Disruption of the human COQ5-containing protein complex is associated with diminished coenzyme Q10 levels under two different conditions of mitochondrial energy deficiency. Biochimica et Biophysica Acta - General Subjects, 1860: 1864-1876, 2016.
2. HC Yen*, CL Lin, BS Chen, CW Chen, KC Wei, ML Yang, JC Hsu, YH Hsu. Alterations of the levels of primary antioxidant enzymes in different grades of human astrocytoma tissues. Free Radical Research, 52: 856-871, 2018.
3. HC Yen*, WY Yeh, SH Lee, YH Feng, SL Yang. Characterization of human mitochondrial PDSS and COQ proteins and their roles in maintaining coenzyme Q10 levels and each other’s stability. Biochim Biophys Acta -Bioenergetics, 1861:148192, 2020.
4. HC Yen*, BS Chen, SL Yang, SY Wu, CW Chang, KC Wei, JC Hsu, YH Hsu, TH Yen, CL Lin*. 2022 February. Levels of coenzyme Q10 and several COQ proteins in human astrocytoma tissues are inversely correlated with malignancy. Biomolecules, 12: 336, 2022.
5. TH Yen, CW Chang, HR Tsai, JF Fu, HC Yen*. Immunosuppressive therapies attenuate paraquat-induced renal dysfunction by suppressing inflammatory responses and lipid peroxidation. Free Radic Biol Med, 191: 249-260, 2022.
6. M Kotepui*, K Kotepui, A Mahittikorn*, HJ Majima, J Tangpong, HC Yen. Association of reduced glutathione levels with Plasmodium falciparum and Plasmodium vivax malaria: a systematic review and meta?analysis. Scientific Reports, 13: 14683, 2023.
7. D Masuda, I Nakanishi*, K Ohkubo, H Ito, K Matsumoto, H Ichikawa, M Chatatikun, WK Klangbud, M Kotepui, M Imai, F Kawakami, M Kubo, H Matsui, J Tangpong, T Ichikawa, T Ozawa, HC Yen, DK St. Clair, HP Indo*, HJ Majima*. Mitochondria play essential roles in intracellular protection against oxidative stress—Which molecules among the ROS generated in the mitochondria can escape the mitochondria and contribute to signal activation in cytosol? Biomolecules, 14: 128, 2024.
8. HC Yen*, CT Hsu, SY Wu, CC Kan, CW Chang, HM Chang, YA Chien, YH Wei, CY Wu. Alterations in coenzyme Q10 status in a cybrid line harboring the 3243A>G mutation of mitochondrial DNA is associated with abnormal mitochondrial bioenergetics and dysregulated mitochondrial biogenesis. Biochimica et Biophysica Acta - Bioenergetics, 1864: 149492, 2024.
* 负责作者(corresponding author)
其他更早论文请见实验室网页
(Please find other previous publications on lab webpage.)