91猫先生

研究室资料

研究主题

本实验室以酵母菌(Saccharomyces cerevisiae)、人类细胞、和小鼠模式进行以下叁大主题之研究：

1. 中草药/天然药物调控DNA修补之分子机转与应用

    臨床研究顯示，許多中草药對於合併放射治療有一定的增效作用，其中一個分子原理，是利用中草药抑制細胞的DNA修补机制，来增加癌症细胞的放射线敏感性。因此研究中药物如何调控DNA修补之分子机制，将有助於设计和发展合併放射治疗的新药物。

2. 中草药對壽命機制之調控與延長壽命/抗肥胖药物之筛选

   目前筛选增加寿命药物的系统极少，本实验室利用一套新颖的筛选系统大量筛选具有延长寿命功效的药物，并研究其分子机制。针对人口老化日趋严重的社会现象，此类型健康食品可用於预防及治疗老化相关的病状，更可望大量减少未来老年人口的医疗资源支出，真正达到健康老化的最终目的。

论文着作

1.    Cheng SF, Lin YT, Wang TH, Leu YL, Hsia KC, Lee WC, Feng CH, Kuo CH, Yang SH, Wang MC, Liu CY, Hu PC, Hsieh YC, Yang CW, Wang SH, Yu JS, Chen CY, Chen CC*. Corylin promotes healthy aging via RAGA-mTOR suppression and sex-dependent activation of SIRT3. (2026) Nature Communications. doi: 10.1038/s41467-026-74184-9.

2.    Liu YY, Chen CC(co-first), Kuo CH, Leu YL, Wang SH. Beneficial Effects of Corylin on Metabolic Dysfunction-Associated Fatty Liver Disease. (2026) Antioxid Redox Signal. 44(16-18):910-927. doi: 10.1177/15230864261443781.

3.    Chen CC, Wang TH, Leu YL, Chan CW, Chen YX, Chu TD, Chen CY. Magnolol suppresses TKI-resistant EGFR-mutant lung cancer by inhibiting EGFR and AXL-cMyc. (2026) Eur J Pharmacol 28;1016:178607. doi: 10.1016/j.ejphar.2026.178607. Epub 2026 Jan 27

4.    Chen CC, Chen CY, Chou LF, Yeh CT, Liu YT, Chu YD, Lin HW, Chen KY, Yu CC, Wang TH. Corylin attenuates oral squamous cell carcinoma progression through c-Myc inhibition. (2025) J Dent Sci. 20(4):2322-2331. doi: 10.1016/j.jds.2025.04.014.

5.    Leu YL, Cheng SF, Wang TH, Feng CH, Chen YJ, Hsieh YC, Lan YH, Chen CC*. Increasing DNA damage sensitivity through corylin-mediated inhibition of homologous recombination. (2024) Biomed Pharmacother.  11;176:116864. doi: 10.1016/j.biopha.2024.116864

6.    Chang TY, Lin MS, Chen CC (co-first), Leu YL, Wang SH. Isoxanthohumol reduces neointimal hyperplasia through the apelin/AKT pathway. (2024) Biochim Biophys Acta Mol Basis Dis.1870(4):167099. doi: 10.1016/j.bbadis.2024.167099. Epub 2024 Feb 28. PMID: 38428686.

7.    Chen CC, Chen CY, Yeh CT, Liu YT, Leu YL, Chuang WY, Shih YH, Chou LF, Shieh TM, Wang TH. Corylin Attenuates CCl4-Induced Liver Fibrosis in Mice by Regulating the GAS6/AXL Signaling Pathway in Hepatic Stellate Cells. (2023) Int J Mol Sci. doi: 10.3390/ijms242316936

8.    Wang TH., Tseng WC., Leu YL., Chen CY., Lee WC., Chi YC., Cheng SF., Lai CY., Kuo CH., Yang SL., Yang SH., Shen JJ., Feng CH., Wu CC., Hwang TL., Wang CJ., Wang SH., Chen CC*. The flavonoid corylin exhibits lifespan extension properties in mouse. (2022) Nature Communications doi:10.1038/s41467-022-28908-2

9.    Chen CC, Chen CY, Cheng SF, Shieh TM, Leu YL, Chuang WY, Liu KT, Ueng SH, Shih YH, Chou LF, Wang TH. Hydroxygenkwanin Increases the Sensitivity of Liver Cancer Cells to Chemotherapy by Inhibiting DNA Damage Response in Mouse Xenograft Models. (2021) Int J Mol Sci. 9;22(18):9766. doi: 10.3390/ijms22189766

10. Wang TH, Chen CC(co-first), Leu YL, Lee YS, Lian JH, Hsieh HL, Chen CY. Palbociclib induces DNA damage and inhibits DNA repair to induce cellular senescence and apoptosis in oral squamous cell carcinoma. (2021) J Formos Med Assoc. 18:S0929-6646(20)30609-4. doi: 10.1016/j.jfma.2020.12.009

11. Tseng WC, Chen CY, Chern CY, Wang CA, Lee WC, Chi YC, Cheng SF, Kuo YT, Chiu YC, Tseng ST, Lin PY, Liou SJ, Li YC, Chen CC*. Targeting HR Repair as a Synthetic Lethal Approach to Increase DNA Damage Sensitivity by a RAD52 Inhibitor in BRCA2-Deficient Cancer Cells. (2021) Int J Mol Sci. Apr 23;22(9):4422. doi: 10.3390/ijms22094422.

12. Chen CC, Kuo CH, Leu YL, Wang SH. Corylin reduces obesity and insulin resistance and promotes adipose tissue browning through SIRT-1 and β3-AR activation. (2021) Pharmacol Res. 27:105291. doi: 10.1016/j.phrs.2020.105291

13. Chen CY, Fang JY, Chen CC(co-first), Chuang WY, Leu YL, Ueng SH, Wei LS, Cheng SF, Hsueh C, Wang TH. 2-O-Methylmagnolol, a Magnolol Derivative, Suppresses Hepatocellular Carcinoma Progression via Inhibiting Class I Histone Deacetylase Expression. (2020) Frontiers in Oncology 10:1319. doi: 10.3389/fonc.2020.01319

14. Chen CY, Chen CC(co-first), Chuang WY, Leu YL, Ueng SH, Hsueh C, Yeh CT, Wang TH. Hydroxygenkwanin Inhibits Class I HDAC Expression and Synergistically Enhances the Antitumor Activity of Sorafenib in Liver Cancer Cells. (2020) Frontiers in Oncology 25;10:216. doi: 10.3389 /fonc.2020.00216

15. Chen CC, Li HY, Leu YL, Chen YJ, Wang CJ, Wang SH. Corylin Inhibits Vascular Cell Inflammation, Proliferation and Migration and Reduces Atherosclerosis in ApoE-Deficient Mice. (2020) Antioxidants 25;9(4) doi: 10.3390/antiox9040275

16. Wang TH, Chen CC(co-first), Huang KY, Shih YM, Chen CY. High levels of EGFR prevent sulforaphane-induced reactive oxygen species-mediated apoptosis in non-small-cell lung cancer cells. (2019) Phytomedicine 64:152926. doi: 10.1016/j.phymed.2019.152926

17. Kuo CH, Leu YL, Wang TH, Tseng WC, Feng CH, Wang SH, Chen CC*. A novel DNA repair inhibitor, diallyl disulfide (DADS), impairs DNA resection during DNA double-strand break repair by reducing Sae2 and Exo1 levels. (2019) DNA repair 82:102690 doi: 10.1016/j.dnarep.2019.102690

18. Chen CC, Lee TY, Leu YL, Wang SH. Pigment epithelium-derived factor inhibits adipogenesis in 3T3-L1 adipocytes and protects against high-fat diet-induced obesity and metabolic disorders in mice. (2019) Translational Research. 210:26-42 doi: 10.1016/j.trsl.2019.04.006

19. Wang TH, Chen CC (co-first), Hsiao YC, Lin YH, Pi WC, Huang PR, Wang TV, Chen CY. Heterogeneous Nuclear Ribonucleoproteins A1 and A2 Function in Telomerase-Dependent Maintenance of Telomeres. (2019) Cancers (Basel). 8;11(3) doi: 10.3390/cancers11030334

20. Chen CC, Chen CY, Wang SH, Yeh CT, Su SC, Ueng SH, Chuang WY, Hsueh C, Wang TH. Melatonin Sensitizes Hepatocellular Carcinoma Cells to Chemotherapy Through Long Non-Coding RNA RAD51-AS1-Mediated Suppression of DNA Repair. (2018) Cancers (Basel). 10;10(9) DOI: 10.3390/cancers10090320

21. Chen CC, Chen CY, Ueng SH, Hsueh C, Yeh CT, Ho JY, Chou LF, Wang TH. Corylin increases the sensitivity of hepatocellular carcinoma cells to chemotherapy through long noncoding RNA RAD51-AS1-mediated inhibition of DNA repair. (2018) Cell Death Dis. 1;9(5):543

22. Chen CC, Huang JS, Wang TH, Kuo CH, Wang CJ, Wang SH, Leu YL. Dihydrocoumarin, an HDAC Inhibitor, Increases DNA Damage Sensitivity by Inhibiting Rad52. (2017) Int J Mol Sci. 18(12),2655. doi: 10.3390/ijms18122655

23. Yeh CJ, Chen CC (co-first), Leu YL, Lin MW, Chiu MM, Wang SH. The effects of artocarpin on wound healing: in vitro and in vivo studies. (2017) Scientific reports; 7(1):15599. doi: 10.1038/s41598-017-15876-7

24. Chen CC, Liang CJ, Leu YL, Chen YL, Wang SH. Viscolin Inhibits In Vitro Smooth Muscle Cell Proliferation and Migration and Neointimal Hyperplasia In Vivo. (2016) PLoS one 11(12): e0168092. doi:10.1371/journal.pone.0168092

25. Chen CC, Lin MW, Liang CJ, Wang SH. The Anti-Inflammatory Effects and Mechanisms of Eupafolin in Lipopolysaccharide-Induced Inflammatory Responses in RAW264.7 Macrophages. (2016) PLoS One. 11(7):e0158662. doi: 10.1371/journal.pone.0158662

26. Wang SH, Lin PY, Chiu YC, Huang JS, Kuo YT, Wu JC, Chen CC*. Curcumin-Mediated HDAC Inhibition Suppresses the DNA Damage Response and Contributes to Increased DNA Damage Sensitivity. (2015) PLoS One. 10(7):e0134110. doi: 10.1371/journal.pone.0134110

27. Hu Z, Chen K, Xia Z, Chavez M, Pal S, Seol JH, Chen CC, Li W, Tyler JK. Nucleosome loss leads to global transcriptional up-regulation and genomic instability during yeast aging. (2014) Genes & Development, 15;28(4):396-408 DOI: 10.1101/gad.233221.113

28. Chen CC. & Tyler, J. K. Selective sensitization of cancer cells to DNA damage by a HAT inhibitor. (2009) Cell Cycle 8(18).

29. Chen CC. & Tyler, J. K. Chromatin assembly signals the end of repair. (2008) Cell Cycle, 7, 3792-7.

30. Chen CC., Carson, J., Feser, J., Tamburini, B., Zabaronick, S., Linger, J. & Tyler, J. K. Acetylation of lysine 56 on histone H3 drives chromatin assembly after repair and signals for the completion of double-strand DNA repair. (2008) Cell, 134, 231-43. OI: 10.1016/j.cell.2008.06.035

专利

1. 陈金銓，吕彦礼，黄聪龙，方嘉佑，潘台龙，

专利名稱：哺乳類SIRT1的活化剂，

台灣专利證號：I556818，

专利期限：2016.11.11-2035.08.06

2. 陈金銓，吕彦礼，王淑慧，专利名稱：哺乳類

专利名稱：TOR的抑制剂，

台灣专利證號：I586351，

专利期限：2017.06.11-2036.02.15

3. 陈金銓, 王淑慧, 吕彦礼

专利名稱：哺乳類SIRT1的活化剂，

美國专利證號：US 9,795,588 B2，

                专利期限：2016.08~ 2036.08

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