91猫先生

课程资讯

学年/学期	112/2
授课教师	李国铭
开课单位	生医所博士班
年级-班别	1-甲
科目代号(学分)	EMM114(2)
课程名称	分子病毒方法学概论
开课序号	61413
主要开课序号
修课学生	本校:&苍产蝉辫;3人；外校:&苍产蝉辫;0人
人数限制	2~60
上课时间地点	星期 开始&苍产蝉辫;/&苍产蝉辫;结束 教室&苍产蝉辫;摆代码说明] 星期一 3 (10:10) ~ 4 (12:00) 每週上课
核心能力
备註	英语授课，惭0860-2

教学大纲

教科书	Lecture slides
教科书ISBN	0000000000
参考书	Molecular cloning : a laboratory manual by Michael Green et al., 4th Edition (2012), Fields Virology: Emerging Viruses by Peter Howley et al., 7th Edition (2020), Molecular Biology by Robert Weaver, 5th Edition (2011), Molecular Virology of Human Pathogenic Viruses by Wang-Shick Ryu (2017)
课程讲义网址
整体教学目标	Aim to let the attendees have a comprehensive understanding of both the traditional and advanced techniques applied in the studies of the molecular virology
教学方法	Lectures, discussion, and student presentation
课程介绍	以濒别肠迟耻谤别的形式介绍实验室进行病毒学研究常用的技术以及方法。除了传统的分生以及生化技术外，也会导入新颖的次世代实验方法。课程会以病毒的分子检测為出发点，当我们遭遇未知的病毒感染源，如何快速辨别可能的病原体？除了传统侦测病毒核酸或是蛋白质表现的分子实验方法，以及以细胞培养為基础进行病毒定量以及抗病毒药物效性的相关实验外，课程还会讨论近年发展出的研究技术如蛋白质、代谢体学，次世代/第叁代定序，以及基因筛选/编辑等新颖的技术及其如何应用於病毒以及病毒宿主间交互作用的相关研究成果
弹性学习	The flexible courses are scheduled to be hold on 2024/06/10 (Mon), and 2024/06/17 (Mon), 10:10-12:00. 1. Physical or online course to be announced 2. Discussion for two Videos: 1. Approaches for using protein-protein interaction networks for biological discovery (European Bioinformatics Institute - EMBL-EBI); 2. Single Cell Sequencing (iBiology Techniques)
成绩考核	Final presentation: 60% Classroom performance and discussion: 30% Attendance: 10%
师生互动时间	Monday 12:00 (pm) to 13:00 Kuo-Ming Lee, g39002007@mail.cgu.edu.tw, x-3727

教学进度

项次	教学主题
1	1. Physical course 2. Topic: Introduction: Methodology in Molecular Virology and Diagnosis 3. Lecturer: Kuo-Ming Lee
2	1. Physical course 2. Topic: Molecular Cloning: DNA Technology and Evolution of the Central Dogma 3. Lecturer: Kuo-Ming Lee
3	1. Physical course 2. Topic: Basic Protein Analysis: Antibody-based Techniques in Molecular, Biochemical, and Cellular Biology 3. Lecturer: Kuo-Ming Lee
4	1. Physical course 2. Topic: Old-91猫先生 Nucleic Acid Analysis: Northern and Southern Blotting, Nuclease-based Analyses, and In Vitro Assays 3. Lecturer: Kuo-Ming Lee
5	1. Physical course 2. Topic: Basic Nucleic Acid Analysis: PCR, RT-PCR and PCR-based Nucleic Acid Amplification Tests (NAAT) 3. Lecturer: Kuo-Ming Lee
6	1. Physical course 2. Topic: Application of CRISPR and Isothermal Amplification in NAATs of Molecular Diagnosis 3. Lecturer: Kuo-Ming Lee
7	1. Physical course 2. Topic: Next and Third-generation Sequencing in the post Genomic Era 3. Lecturer: Kuo-Ming Lee
8	1. Physical course 2. Topic: Bioinformatic and Evolutionary Analysis: Tools in Epidemiology of SARS-CoV-2 3. Lecturer: Yu-Nong Gong
9	1. Physical course 2. Topic: Cell Culture and Various Nucleic Acid Delivery Systems 3. Lecturer: Kuo-Ming Lee
10	1. Physical course 2. Topic: Derivatives from RNA Viruses as Prospective Tools for Clinical and Basic Research 3. Lecturer: Kuo-Ming Lee
11	1. Physical course 2. Topic: mRNA Vaccine and other Nucleic Acid Therapeutics 3. Lecturer: Kuo-Ming Lee
12	1. Physical course 2. Topic: Virus Propagation, Viral Titer Measurement and Antiviral Assay 3. Lecturer: Yu-An Kung
13	1. Physical course 2. Topic: Serological Tests in Molecular Virology 3. Lecturer: Yu-An Kung
14	1. Physical course 2. Topic: CRISPR/Cas System in the Genetic Screening 3. Lecturer: Kuo-Ming Lee
15	1. Physical course 2. Topic: Other Genetic Screening strategies for Virus-Host Interactions: RNAi and Haploid Screening 3. Lecturer: Kuo-Ming Lee
16	1. Physical course 2. Topic: Final Presentation and Group Discussion 3. Lecturer: Kuo-Ming Lee

先修课程

本課程沒有先修课程規定

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