91猫先生

研究方向及研究室特色：

广东住血线虫是导致嗜伊红性脑膜炎的主要病原体。人类不慎感染后，虫体会侵犯人的中枢神经系统，引发脑部免疫反应，造成嗜酸性白血球聚集及细胞激素释放，导致致命病变。本研究室主要探讨宿主脑部与广东住血线虫感染之间的相关机制调控。

1. 虫体与宿主之间相互作用

针对广东住血线虫排泄分泌产物进行研究，包含microRNA与蛋白质，比如disulfide-isomerases、calreticulin、aspartic protease与annexin等，探討与宿主分子機轉之關聯性。此外，目前也以體學針對广东住血线虫不同龄期幼虫之基因表现差异性进行分析，希望找出与蟲體感染及致病之重要基因，以供後續研究。

2. 感染后宿主分子机转

研究广东住血线虫排泄分泌产物引起宿主脑部细胞之分子机转，并探讨Sonic hedgehog訊號路徑在其中參与之角色，釐清各讯号路径之相互关係。

3. 治疗策略之评估

目前临床上治疗广东住血线虫症主要以症狀与支持療法為主，致今還未研發出最有效治療方法。本研究室欲以細胞及實驗動物層次，評估小鼠腦部細胞在广东住血线虫感染后，相关药物之治疗效果，提供未来广东住血线虫症治疗之参考。

最近五年所发表论文：

1. Jhan KY, Cheng CJ, Chou CJ, Jung SM, Lai GJ, Chen KY*, Wang LC*. (2022) Improvements of cognitive functions in mice heavily infected by Angiostrongylus cantonensis after treatment with albendazole, dexamethasone, or co-therapy. J Microbiol Immunol Infect. S1684-1182(22)00058-5. doi: 10.1016/j.jmii.2022.04.004.
2. Chen KY, Chen YJ, Cheng CJ, Jhan KY, Wang LC. (2022) Benzaldehyde Attenuates the Fifth Stage Larval Excretory-Secretory Product of Angiostrongylus cantonensis-Induced Injury in Mouse Astrocytes via Regulation of Endoplasmic Reticulum Stress and Oxidative Stress. Biomolecules. 12(2):177. doi: 10.3390/biom12020177.
3. Jhan KY, Cheng CJ, Jung SM, Lai YJ, Chen KY*, Wang LC*. (2021) Co-Therapy of Albendazole and Dexamethasone Reduces Pathological Changes in the Cerebral Parenchyma of Th-1 and Th-2 Dominant Mice Heavily Infected with Angiostrongylus cantonensis: Histopathological and RNA-seq Analyses. Biomolecules. 11(4):536.
4. Chen KY, Chen YJ, Cheng CJ, Jhan KY, Chiu CH, Wang LC. (2021) 3-Hydroxybenzaldehyde and 4-Hydroxybenzaldehyde enhance survival of mouse astrocytes treated with Angiostrongylus cantonensis young adults excretory/secretory products. Biomed J. 44(6 Suppl 2):S258-S266. doi: 10.1016/j.bj.2020.11.008.
5. Chen KY, Cheng CJ, Cheng CC, Jhan KY, Chen YJ, Wang LC. (2020) The excretory/secretory products of fifth-stage larval Angiostrongylus cantonensis induces autophagy via the Sonic hedgehog pathway in mouse brain astrocytes. PLoS Negl Trop Dis. 14(6):e0008290.
6. Chen KY, Chen YJ, Cheng CJ, Jhan KY, Wang LC. (2020) Excretory/secretory products of Angiostrongylus cantonensis fifth-stage larvae induce endoplasmic reticulum stress via the Sonic hedgehog pathway in mouse astrocytes. Parasit Vectors. 13(1):317.
7. Jhan KY, Lai GJ, Chang PK, Tang RY, Cheng CJ, Chen KY*, Wang LC*. (2020) Angiostrongylus cantonensis causes cognitive impairments in heavily infected BALB/c and C57BL/6 mice. Parasit Vectors. 13(1):405.
8. Chen KY, Lu PJ, Cheng CJ, Jhan KY, Yeh SC, Wang LC. (2019) Proteomic analysis of excretory-secretory products from young adults of Angiostrongylus cantonensis. Mem Inst Oswaldo Cruz. 114:e180556.
9. Wang TY*, Chen KY*, Jhan KY, Li CH, Jung SM, Wang LC. (2018) Temporal-spatial expressions of interleukin-4, interleukin-10, and interleukin-13 in the brains of C57BL/6 and BALB/c mice infected with Angiostrongylus cantonensis: An immunohistochemical study. J Microbiol Immunol Infect. S1684-1182(18)30173-7.
10. Chen KY, Wang LC. (2017) Stimulation of IL-1β and IL-6 through NF-κB and sonic hedgehog-dependent pathways in mouse astrocytes by excretory/secretory products of fifth-stage larval Angiostrongylus cantonensis. Parasit Vectors. 10(1):445.
11. An YC, Sung CC, Wang CC, Lin HC, Chen KY, Ku FM, Chen RM, Chen ML, Huang KY. (2017) Molecular Identification of Diphyllobothrium latum from a Pediatric Case in Taiwan. Korean J Parasitol. 55(4):425-428.
12. Chen KY, Yen CM, Hwang KP, Wang LC. (2017) Enterobius vermicularis infection and its risk factors among pre-91猫先生 children in Taipei, Taiwan. J Microbiol Immunol Infect. S1684-1182(17)30117-2.
13. Chen KY, Chiu CH, Wang LC. (2017) Anti-apoptotic effects of Sonic hedgehog signalling through oxidative stress reduction in astrocytes co-cultured with excretory-secretory products of larval Angiostrongylus cantonensis. Sci Rep. 7:41574.