91猫先生

研究方向及研究室特色

我们实验室主要研究细胞分化的过程。在发育过程中，胚胎细胞藉由分化过程来形成各式功能的细胞。分化作用也会在成体中发生，并对成体组织代谢及恆定极為重要。因此，研究细胞分化不只有助於了解及治疗因细胞功能不足而产生的先天性疾病,且直接有助於未来在医学上产生新生细胞来修补受损组织。

我们主要用果蝇这个模式生物来研究在活体中，基因如何调控细胞分化。实验室中有两个主要方向：(1)探讨神经母细胞的形成过程中，原神经蛋白如何活化下游基因来促进母细胞分化。我们目前着重於探讨核内肌动蛋白和原神经蛋白之间的关係。(2)成体干细胞 (adult stem cell) 自我新生的调控在 维持组织恆定中扮演 重要功能 。自我新生能力太强时, 细胞分化会被抑制。 反之, 过早进入细胞分化会降低干细胞的自我新生。我们实验室最近的研究在探讨成体中的配子干细胞如何受到BMP 或其它讯息传导路径的影响来维持在自我新生及细胞分化中的协调。

(A)果蝇的翅膀 (B)感觉神经刚毛(箭头)位於果蝇前翅脉边缘，负责感觉外界机械性刺激 (C)果蝇成体testis前端，围绕在hub(中心白色细胞)周围的细胞為成体配子干细胞(箭头)

近五年计划

科技部：

2015/8/1-2018/7/31 Arp6 及 H2Av 在果蝇外感觉器官分佈及发育的功能

2018/8/1-2019/7/31 bHLH 原神经蛋白在神经母细胞中对核醣体的生化合成的调控

2019/8/1-2020/7/31果蝇神经形成中肌动蛋白相关蛋白对神经前驱蛋白质的转译调控   

2021/8/1-2023/7/31 异质性的核醣体和优先选择转译调控机制对於果蝇原神经蛋白调控之神经发育的影响

2015/7/1-2016/2/29 【104大专生】探讨细菌感染及免疫反应在公果蝇性腺癌化的过程中所扮演的角色(学生姓名：张婷宇)

2018/7/1-2019/2/28【107大专生】核仁蛋白Dbp73D与NS2在前驱原神经蛋白所调控的神经发育中之功能(学生姓名：陈文华)   【108大专生】

2019/7/1-2020/2/28【108大专生】MicroRNA in suppression of Tumor Necrosis Factor in testis smooth muscles in Drosophila(学生姓名：吴文婷)   

长庚医院：

2016/6/1-2019/5/31 在雄性果蝇性腺中，JNK讯息传递鍊调节囊干细胞恆定的分子机制

2020/10/1-2022/9/30 利用果蝇神经新生為模式，研究及筛选神经发育疾病候选基因

最近五年所发表论文

Publication list

1.        Yun-Ling Hsiao, Hui-Wen Chen, Kuan-Han Chen, Bertrand Chin-Ming Tan, Chia-Hsiang Chen, and Haiwei Pi (2023). Actin-related protein 6 facilitates proneural protein-induced gene activation for rapid neural differentiation. Development, March 1; 150 (5): dev201034 (SCI: 6.862; Rank: 12.8% (5/39) in Developmental Biology). This paper is selected as a “Research Highlight” in Development journal.

2.        Hsun Li, Hsin-Ho Sung, Yi-Chun Huang, Ying-Ju Cheng, Hsiao-Fong Yeh, Haiwei Pi, Edward Giniger, and Cheng-Ting Chien (2022). Fringe-positive Golgi outposts unite temporal Furin 2 convertase activity and spatial Delta signal to promote dendritic branch retraction. Cell Reports, Sep 20; 40(12):111372. (SCI: 9.995; Rank: 17% (33/195) in Cell biology)

3.        Pei-Yu Wang, Archan Chakraborty, Hsin-Ju Ma, Jhen-Wei Wu, Anna C.-C. Jang, Wei-Cheng Lin1, Hai-Wei Pi, Chau-Ting Yeh, Mei-Ling Cheng, Jau-Song Yu and Li-Mei Pai (2022). Drosophila CTP synthase regulates collective cell migration by controlling the polarized endocytic cycle. Development, Aug 15; 149 (16): dev200190. (SCI: 6.862; Rank: 12.8% (5/39) in Developmental Biology)

4.        Yi Chieh Chang, Hsin Tu, To?Wei Huang, Bo?Wen Xu & Haiwei Pi* (2020). Upregulated TNF/Eiger signaling mediates stem cell recovery and tissue homeostasis during nutrient resupply in Drosophila testis. Scientific Reports, Jul, 15; 10 (1): 11674 (SCI: 4.997; Rank: 25.6% (19/74) in Multidisciplinary Science)

5.        Kun-Yang Lin, Wen-Der Wang, Chi-Hung Lin, Elham Rastegari, Yu-Han Su, Yu-Tzu Chang, Yung-Feng Liao, Yi-Chieh Chang, Haiwei Pi, Bo-Yi Yu, Shu-Hwa Chen, Chung-Yen Lin, Mei-Yeh Lu, Tsu-Yi Su, Fei-Yang Tzou, Chih-Chiang Chan & Hwei-Jan Hsu (2020). Piwi reduction in the aged niche eliminates germline stem cells via Toll-GSK3 signaling. Nature Communications, 11 (1):3147. (SCI: 17.694; Rank: 8% (6/74) in Multidisciplinary Science).

6.        Yi Chieh Chang, Hsin Tu, Jing-Yi Chen, Ching-Chin Chang, Shu Yuan Yang, Haiwei Pi* (2019). Reproduction disrupts stem cell homeostasis in testes of aged male Drosophila via an induced microenvironment. PLOS Genetics, 15 (7): e1008062. (SCI: 6.02; Rank: 15.4% (27/175) in Genetics and Heredity). This paper is featured in PLOS Genetics by an accompanying Perspective article “ Irreversible effects of youthful choices in aged adults”.

7.        Yang SY*, Chang YC, Wan YH, Whitworth C, Baxter EM, Primus S, Pi H, Van Doren M*. (2017). Control of a Novel Spermatocyte-Promoting Factor by the Male Germline Sex Determination Factor PHF7 of Drosophila melanogaster. GENETICS, 206(4),1939-1949. (SCI: 4.4; Rank: 32% (56/175) in Genetics and Heredity)

8.        Chien-Hsiang Wang, Yi-Chun Huang, Pei-Yi Chen, Ying-Ju Cheng, Hsiu-Hua Kao, Haiwei Pi, Cheng-Ting Chien (2017). USP5/Leon deubiquitinase confines postsynaptic growth by maintaining ubiquitin homeostasis through Ubiquilin . eLife, 10(6),e26886. (SCI: 8.713; Rank: 8.5% (8/94) in Biology)

9.        Shih-Han Kao, Chen-Yuan Tseng, Chih-Ling Wan, Yu-Han Su, Chang-Che Hsieh, Haiwei Pi, and Hwei-Jan Hsu (2015). Aging and insulin signaling differentially control normal and tumorous germline stem cells. Aging Cell, 14(1): 25–34. (SCI: 11.005; Rank: 15.3% (30/195) in Cell Biology)

10.     Yi-Chun Huang,Yu-Nung Lu,June-Tai Wu,Cheng-Ting Chien, Haiwei Pi* (2014). The COP9 Signalosome Converts Temporal Hormone Signaling to Spatial Restriction on Neural Competence. PLOS Genetics, 10(11),1-13. (SCI: 6.02; Rank: 15.4% (27/175) in Genetics and Heredity)