郭敏玲 / Ming-Ling Kuo
职称： 教授
现职： 长庚大学生物医学研究所所長
电话： 3319/3320
学歷： 美国罗格斯大学博士
专长领域： 免疫学
个人网页：
个人网页
研究方向及研究室特色
本实验室以过敏相关疾病為研究主轴，研究疾病的分子机制及开发新疗法。过敏与气喘疾在已开发国家盛行率愈来愈高，病严重影响人民的健康与消耗许多医疗资源，这类疾病是由多个遗传与环境因子引起体内免疫系统不平衡反应所致，我们的研究重心主要在於深入探讨可以调控过敏病的分子与细胞间反应，期许应用於研发新的防治方式。
气喘病之基因治疗：基於气喘常為第二型T 辅助细胞过度反应所致，嚐试利用病毒载体携带抗发炎分子或第一型T 辅助细胞相关之细胞激素基因，经由气道施予疾病模式小鼠，用以平衡过度活化的第二型T 辅助细胞免疫反应；或是携带可干扰第二型T 辅助细胞反应的基因片段，评估其抑制气喘疾病各项指标的可行性。
药物或是抗体引发T 調節細胞的發展：近年，如何調節過度活化的免疫反應成為免疫学的熱門課題，我們亦著手研究與表觀遺傳學的基因表達調控相關藥物、或是可改變T 辅助细胞族群活化功能的特殊抗体等，对人类周边血球中的被激活T 辅助细胞或是气喘疾病模式小鼠的免疫调节功能。在逐步研究这些药物如何调控气喘疾病发生的分子机制过程中，可以进一步发展运用这些药物或抗体来治疗气喘疾病。
探讨可治疗气喘中药之科学证据：以临床使用疗效佳之方剂如「定喘汤」，利用气喘动物模式来研究中药使用之剂量、给药时机、疗效机制及安全性，做為分离出有效成分之基础；也和中、西医临床医师合作，完成疗效评估。在中医药科学化方面，则将注重中药作用於细胞讯号传递系统之机制，并搭配以中草药材中的纯化药物，来探讨并分析各药物对周边淋巴球细胞活性的调控或是对气喘疾病模式小鼠的抑制发炎功能。
脐带血淋巴细胞活性研究：比起骨髓，移植脐带血中造血系统，有材料取得的方便性与排斥性低的优点。所以本实验室的另外一个研究主题，是和长庚儿童医院医师合作，探讨以不同细胞激素组合对脐带血中 T 辅助细胞及自然杀手细胞之复製、死亡及活性的调控，或是评估不同免疫抑制剂对细胞功能的影响，可以对脐带血移植时的免疫反应，有更多的了解。
论文与着作
Pubication *: Correspondence author ?: Co-correspondence author #: equal contribution
2021
1. Chen L-C, Tseng H-M, Kuo M-L, Chiu C-Y, Liao S-L, Su K-W, Tsai M-H, Hua M-C, Lai S-H, Yao T-S, Yeh K-W, Wu A-H, Yu H-Y, Huang J-L, Huang S-K. 2021(). Levels of 15-HETE and TXB in exhaled breadth condensates as markers for diagnosis of childhood asthma and its therapeutic outcome. Pediatr. Allergy Immunol. (DOI: 10.1111/pai.13587) Online ahead of print. June 27 2021.
2. Chang-Chien J, Huang J-L, Tsai H-J, Wang S-L, Kuo M-L?. Yao T-C?. 2021 (Oct). Particulate matter causes telomere shortening and cellular senescence in human lung epithelial cells. Ecotox. Environ. Safe. 2021, 222: 112484-112492. (DOI: 10.1016/j.ecoenv.2021.112484)
3. Chen J-C, Chan C-C, Ting N-C, Kuo M-L*. 2021 (June). Allergen exposure in murine neonates promoted the development of allergic lungs. Biomedicines. 2021, 9(6): 688-703. (DOI: 10.3390/biomedicines9060688)
4. Huang Y-A, Chen J-C, Wu C-C, Hsu C-W, Ko Albert M-S, Chen L-C, Kuo M-L*. 2021 (June). Reducing lung ATP levels and alleviating asthmatic airway inflammation through adeno-associated viral vector-mediated CD39 expression. Biomedicines. 2021, 9(6): 656-669. (DOI: 10.3390/biomedicines9060656)
5. Chen J-C, Ou L-S, Kuo M-L, Tseng L-Y, Chang H-L, Chen S-C, Chui C-H. 2021 (March 01). Fetal macrophages exposed to Salmonella antigens elicit protective immunity against overwhelming Salmonella challenge in a murine model. Biomedicines. 9(3): 245-260. (DOI: 10.3390/biomedicines9030245)
2020
1. Chen Y-W, Huang M-Z, Vhen C-L, Kuo C-Y, Yang C-Y, Ni-Chiang C, Chen Y-Y, Hsieh C-M, Wu H-Y, Kuo M-L, Chiu C-H, Lai C-H*. 2020 (Aug 4). PM2.5 impairs macrophage functions to exacerbate pneumococcus-induced pulmonary pathogenesis. 2020. Part. Fibre Toxicol. 2020. 17:37-51. (DOI: 10.1186/s12989-020-00362-2.)
2. Khan M, Huang Y-A, Kuo C-Y, Lin T, Lu C-H, Chen L-C, Kuo M-L*. 2020. (July 30). Blocking pannexin1 reduces airway inflammation in a murine model of asthma. Am. J. Transl. Res. 2020. 12(7): 4074–4083. (PMCID: PMC7407700)
3. Ma Janson, Chan C-C, Huang W-C, Kuo M-L*. 2020 (June 08). Berberine inhibits pro-inflammatory cytokine-induced IL-6 and CCL11 production via modulation of STAT6 pathway in human bronchial epithelial cells. Int. J. Med. Sci. 2020.17(10): 1464- 1473. (DOI: 10.7150/ijms.45400)
4. Chen J-C, Ou L-S, Kuo M-L, Tseng L-Y, Chang H-L. 2020 (June 18). Fetal exposure to oncoantigen elicited antigen-specific adaptive immunity against tumorigenesis. J. Immunother. Cancer. 2020. 8(1): e000137. (DOI: 10.1136/jitc-2019-000137)
5. Lu C-H, Chen C-M, Ma J,Wu C-J, Chen L-I, Kuo M-L*. 2020 (Apr 27). DNA methyltransferase inhibitor alleviates bleomycin-induced pulmonary inflammation. Int. Immunopharmacol. 2020. 84:106542. (DOI:10.1016/j.intimp.2020.106542)
6. Lin S-J *, Hsu C-Y, Kuo M-L, Lee P-T, Hsiao H-S, Chen J-Y*. 2020 (Apr 3). Phenotypic and functional characterization of natural killer cells in rheumatoid arthritis-regulation with interleukin-15. Sci. Rep. 2020. 10:5858-5866. (DOI: 10.1038/s41598-020-62654-z)
7. Chang Y-L, Lo H-Y, Cheng S-P, Chang K-T, Lin X-F, Lee S-P, Ma Janson, Kuo M-L, Hsieh M-F*, Chin C-K*. 2020 (Feb).Therapeutic efficacy of subcutaneous and intraperitoneal injections of a single dose of human umbilical mesenchymal stem cells in acute and chronic colitis in a mouse model. J. Med. Biol. Eng. 2020. 40:82-90. (DOI: 100.1007/s40846-019-00494-7)
2014
1. Liou C-J, Cheng P-Y, Huang W-C, Chan C-C, Chen M-C, Kuo M-L, Shen J-J*. 2014 (Nov). Oral lovastatin attenuates airway Inflammation and mucus secretion in ovalbumin-induced asthmatic mice. Allergy Asthma Immunol. Res. 2014. 6(6):548-557. (DOI: 10.4168/aair.2014.6.6.548)
2. Lin S-J, Lu C-H, Yan D-C, Lee P-T, Hsiao H-S, Kuo M-L*. 2014 (Oct) Expansion of regulatory T cells from umbilical cord blood and adult peripheral blood CD4+CD25+ T cells. Immunol. Res. 2014. 60 (1):105-111. (DOI: 10.1007/s12026-014-8488-1)
3. Chen J-C, Ou L-S, Yu H-Y, Kuo M-L, Chang P-Y, Chang H-L. 2014 (Jul). Recipients with in utero induction of tolerance upregulated MHC class I in the engrafted donor skin. Dis Markers. 2014:531092. (DOI: 10.1155/2014/531092)
4. Huang W-C #, Chan C-C #, Wu S-J, Chen L-C, Shen J-J, Kuo M-L, Chen M-C, Liou C-J. 2014 (Jan). Matrine attenuates allergic airway inflammation and eosinophil infiltration by suppressing eotaxin and Th2 cytokines production in asthmatic mice. J. Ethnopharmacol. 2014. 151 (1): 470-477. (DOI:10.1016/j.jep.2013.10.065)
Review articles:
1. Lin S-J, Yan D-C, Lee Y-C, Hsiao H-S, Lee P-T, and Kuo
M-L*. 2012 (Feb). Umbilical cord blood immunology - revelance to stem
cell transplantation. Clin. Rev. Allergy Immunol. 42 (1):
45-57.
(DOI: 10.1007/s12016-011-8289-4, Dec 2011)
2. Lin S-J and Kuo M-L. 2011 (Nov). Cytotoxic
function of umbilical cord blood natural killer cells: relevance to adoptive
immunotherapy. Pediatr. Hematol. Oncol. 28 (8): 640-646.
(DOIi:10.3109/08880018.2011.613092, Oct 4, 2011)
3. Lin S-J, Yan D-C, Lee Y-C, and Kuo M-L*.
2008 (Nov). Role of interleukin
(DOI: 10.1080/08830180802317940).-15 in umbilical cord blood
transplantation. Int. Rev. Immunol. 27 (6): 518-531.
Book chapters:
1. Lin S-J, Lee P-T, Kuo M-L. Cytokine activation of natural killer cells. Ch19. pp in Cancer Vaccines: Methods and Protocols, Methods in Molecular Biology, Vol 1139. Springer Science+Business Media, New York, NY, USA. DOI:10.1007/978-1-4939-0345-0_19.
2. Lin S-J, Lee Y-C, Yan D-C, Kuo M-L. Natural killer cells in allergy. Ch38, pp 511-517, in Lotze M and Thomson A, eds. Natural Killer Cells, Basic Science and Clinical Application, Elsevier, San Diego, CA, USA. 2009.
Perspectives or comments:
1. Lin H-H, Kuo M-L. 2012 (Mar). The 2011 Nobel Prize in Physiology or Medicine. Chang Gung Med. J. 35 (2): 93-95.
2. Kuo M-L*. 2009. Attempts to bridge the gap between scientific mechanism-based medicine and Chinese herbal extract treatment for asthma. in press, Pediatr. Neonatol. 49 (4): 101-102. (editorial comments, not SCI)