91猫先生

研究室特色

分子调控暨生物资讯研究室之主要研究目标為以单细胞生物阴道鞭毛虫作实验材料，探讨细胞寿命延长及化的调控机转。研究特色是利用生物资讯整合各种经由高通量分析平臺所产生之数据对基因体、转译体，蛋白质体作為基础，并从此等资料中进行探磡，发掘可能调命单细胞原生动物寿命延长及化的机转。

研究方向：阴道鞭毛虫微小蛋白调控缺铁反应之研究

微小蛋白质是由少於一百个胺基酸所组成的蛋白质，最近的研究显示这群蛋白质参与许多细胞内反应，如肿瘤生成、压力反应及讯息传递等。然而，因為传统的基因註解方式将一百个胺基酸的长度作為一个阀值，将转译出短於一百个胺基酸的开放阅读框排除，导致微小蛋白一直是被忽略的一群。

基於近年发展成熟的次世代定序技术，其应用广泛，不仅让微小蛋白质得已被鑑定出来，更藉此能获得更全面的基因表现资讯。然而截至目前為止，微小蛋白质尚未在任何原虫中被发现。本研究室将利用阴道鞭毛虫作為一个模式生物，鑑定并探讨微小蛋白质在缺铁压力下所扮演的角色。阴道鞭毛虫是造成人类非病毒性、盛行率最高的性接触传染病阴道鞭毛虫症的病原体。铁离子对於调控阴道鞭毛虫细胞内能量代谢、氧化还原平衡及氢化酶体质量等相当重要，因此阴道鞭毛虫如何在缺铁情况下生存是相当重要的课题。经由全基体开放阅读框、第二代/第叁代高通量定序及蛋白质体学整合性生物资讯分析，已初步确认在阴道鞭毛虫的转录体及蛋白体中可发现新的微小蛋白。这初步研究结果开启了一个全新的研域。因此未来计画的目标為研究阴道鞭毛虫的微小蛋白质在缺铁情况的功能性角色，并且建立一套针对微小蛋白质的实验及分析平台。

最近五年所发表论文

1.     Cheng WH, Huang KY, Ong SC, Ku FM, Huang PJ, Lee CC, Yeh YM, Lin R, Chiu CH. Tang P. (2020, Sep). Protein cysteine S-nitrosylation provides reducing power by enhancing lactate dehydrogenase activity in Trichomonas vaginalis under iron deficiency. Parasites & Vectors, 13(1): 477-491. (SCI, 3/23, TROPICAL MEDICINE). MOST 107-2320-B-182-021-MY3. 本人為通讯作者.

2.     Lin HC, Chu LJ, Huang PJ, Cheng WH, Zheng YH, Huang CY, Hong SW, Chen LC, Lin HA, Wang JY, Chen RM, Lin WN, Tang P, Huang KY. (2020, Jun). Proteomic signatures of metronidazole-resistant Trichomonas vaginalis reveal novel proteins associated with drug resistance. Parasites & Vectors, 13(1):274. (SCI, 3/23, TROPICAL MEDICINE).

3.     Huang KY, Ong SC, Wu CC, Lin HC, Fang YK, Cheng WH, Huang PJ, Chiu CH, Tang P. (2019, Aug). Metabolic reprogramming of hydrogenosomal amino acids in Trichomonas vaginalis under glucose restriction. J Microbiol Immunol Infect., 52(4):630-637. (SCI, 33/93, INFECTIOUS DISEASES). MOST 105-

2320-B-016-019-MY2. 本人為通讯作者.

4.     Lee CC, Chen WH, Huang PJ, Yeh YM, Chen SY, Chiu CH, Tang P. (2019, Jul). Pathogenic Protist Transmembranome database (PPTdb): A web-based platform for searching for and analysis of protist transmembrane proteins. BMC Bioinformatics 20(S13):382. (SCI, 9/59, MATHEMATICAL & COMPUTATIONAL BIOLOGY). MOST 107-2320-B-182-021-MY3. 本人為通讯作者.

5.     Huang KY, Chen RM, Lin HC, Lin HA, Lin WN, HuangPJ, Chiu CH, Tang P (2019, Apr). Potential role of autophagy in proteolysis in Trichomonas vaginalis. J Microbiol Immunol Infect., 52(2), 336-344. (SCI, 33/93, INFECTIOUS DISEASES). MOST 105-2320-B-016-019. 本人為通讯作者.

6.     Huang PJ, Lee CC, Chiu LY, Huang KY, Yeh YM, Yang CY, Chiu CH, Tang P. (2018, May). VAReporter: Variant reporter for cancer research of massive parallel sequencing. BMC Genomics 19(S2):86, 19(S2):86. (SCI, 43/156, BIOTECHNOLOGY & APPLIED MICROBIOLOGY). 本人為通讯作者.

7.     Huang PJ, Chiu LY, Lee CC, Yeh YM, Huang KY, Chiu CH, Tang P (2018, Jan). mSignatureDB: a database for deciphering mutational signatures in human cancers. Nucleic Acids Research, 46(D1), D964-D970. (SCI, IF 11.5201, 15/297,

BIOCHEMISTRY & MOLECULAR BIOLOGY). 本人為通讯作者.

8.     Chen TW, Gan RC, Fang YK, Chien KY, Liao WC, Chen CC, Wu TH, Chang IY, Yang C, Huang PJ, Yeh YM, Chiu CH, Huang TW, Tang P (2017, Sep). FunctionAnnotator, a versatile and efficient web tool for non-model organism annotation. Scientific Reports, 7(1):10430. (SCI, IF 4.259, 10/64, MULTIDISCIPLINARY SCIENCES). 本人為通讯作者.

9.     Cheng WH, Huang KY, Huang PJ, Lee CC, Yeh YH, Ku FM, Lin R, Cheng ML, Chiu CH Tang P (2017, Sep). γ-Carboxymuconolactone decarboxylase: a novel cell cycle-related basal body protein in the early branching eukaryote Trichomonas vaginalis. Parasites & Vectors, 10(1):443. (SCI, IF 3.163, 2/20, TROPICAL MEDICINE). MOST 104-2320-B-182-025-MY3. 本人為通讯作者.

10.   Sablok G, Chen TW, Lee CC, Varotto C, Gan RC, Nayak KC, Huang PJ, Tang P (2017, Jun). ChloroMitoCU: Codon patterns across organelle genomes for functional genomics and evolutionary applications.. DNA Research, 24(3):327- 332. (SCI, IF 5.415, 23/171, GENETICS & HEREDITY). 本人為通讯作者.

11.   Gan RC, Chen TW, Wu TH, Huang PJ, Lee CC, Yeh YM, Chiu CH, Huang HD, Tang P. (2016, Dec). PARRoT- A homology-based strategy to quantify and compare RNA-Sequencing from non-model organisms. BMC Bioinformatics, 17(S19):513. (SCI, IF 2.435, 10/56, MATHEMATICAL & COMPUTATIONAL BIOLOGY). 本人為通讯作者.

12.   Fang YK, Chien KY, Huang KY, Cheng WH, Ku FM, Lin R, Chen TW, Huang PJ, Chiu CH, Tang P (2016, Nov). Responding to a zoonotic emergency with multi-omics research: Pentatrichomonas hominis hydrogenosomal protein characterization with use of RNA sequencing and proteomics. OMICS: A Journal of Integrative Biology, 20(11): 662-669. (SCI, IF 2.896, 27/254, PHARMACOLOGY & PHARMACY). 本人為通讯作者.

13.   Huang KY, Ku FM, Cheng WH, Lee CC, Huang PJ, Chu LCJ, Cheng CC, Fang YK, Wu HH, Tang P (2015, Nov). Novel insights into the molecular events linking to cell death induced by tetracycline in the amitochondriate protozoan Trichomonas vaginalis. Antimicrob. Agents Chemother, 59(11):6891-903. (SCI, IF 4.476, 27/254, PHARMACOLOGY & PHARMACY). MOST 101-2320-B- 182-025-MY3. 本人為通讯作者.

14.   Chen TW, Gan RC, Chang YF, Liao WC, Wu TH, Lee CC, Huang PJ, Lee CY, Chen YYM, Chiu CH, Tang P (2015, Aug). Is the whole greater than the sum of its parts? De novo assembly strategies for bacterial genomes based on paired-end sequencing. BMC Genomics, 16:648. (SCI, IF 3.986, 26/162, BIOTECHNOLOGY & APPLIED MICROBIOLOGY). 本人為通讯作者.

15.   Chen WH, Huang KY, Huang PJ, Hsu JH, Fang YK, Chiu CH, Tang P (2015,

Aug). Nitric oxide maintains cell survival of Trichomonas vaginalis upon iron depletion. Parasites & Vectors, 8, 393. (SCI, IF 3.163, 2/20, TROPICAL MEDICINE). MOST 104-2320-B-182-025-MY3. 本人為通讯作者.

16.   Huang PJ, Lee CC, Yeh YM, Huang KY, Gan RC, Chen TW, Lee CY, Yang ST, Liao CS, Liu H, Tang P. (2015, Feb). Vanno: A Visualization-aided Variant Annotation Tool. Human Mutation, 36(2):167-74. (SCI, IF 5.144, 26/167;

GENETICS & HEREDITY). 本人為通讯作者.

17.   Huang PJ, Lee CC, Tan BCM, Yeh YM, Chu JLC, Chen TW, Chang KP, Gan RC, Liu H, Tang P (2015, Jan). CMPD: cancer mutant proteome database. Nucleic Acids Research, 43:D849-55. (SCI, IF 11.561, 10/292, BIOCHEMISTRY & MOLECULAR BIOLOGY). MOST 103-2632-B-182-001. 本人為通讯作者.