91猫先生

长庚学学术能量集萃

研究方向及研究室特色

研究题目: Drug discovery against RNA viruses 

研究方向:

1.我们实验室从事抗病毒中药与小分子药物的开发，希望从临床中药复方或单味中药，来开发与筛选具抗流行性感冒病毒、新冠病毒与肠病毒的抑制药物，并研究其机转，以因应可能爆发之疫情。

2. 利用定量蛋白质体学技术/高通量次世代定序，鑑定药物之靶标蛋白质，透过miRNA/lncRNA表现差异，诱发讯号传递，了解宿主-病毒的相互调控，并研究其相关的抗病毒机制。

最近五年所发表论文

(#first author, *corresponding author, 2016-2021)

1. Tang, W. F., Huang, R. T., Chien, K. Y., Huang, J. Y., Lau, K. S., Jheng, J. R., Chiu, C. H., Wu, T. Y., Chen, C. Y., and Horng*, J. T. (2016) Host MicroRNA miR-197 Plays a Negative Regulatory Role in the Enterovirus 71 Infectious Cycle by Targeting the RAN Protein. Journal of virology 90, 1424-1438

2. Jheng, J. R., Wang, S. C., Jheng, C. R., and Horng*, J. T. (2016) Enterovirus 71 induces dsRNA/PKR-dependent cytoplasmic redistribution of GRP78/BiP to promote viral replication. Emerg Microbes Infect 5, e23

3. Ho, J. Y., Chern, J. H., Hsieh, C. F., Liu, S. T., Liu, C. J., Wang, Y. S., Kuo, T. W., Hsu, S. J., Yeh, T. K., Shih, S. R., Hsieh, P. W., Chiu, C. H., and Horng*, J. T. (2016) In vitro and in vivo studies of a potent capsid-binding inhibitor of enterovirus 71. The Journal of antimicrobial chemotherapy 71, 1922-1932

4. Lin, T. J., Lin, C. F., Chiu, C. H., Lee, M. C., and Horng*, J. T. (2016) Inhibition of endosomal fusion activity of influenza virus by Rheum tanguticum (da-huang). Sci Rep 6, 27768

5. Hsieh, C. F., Chen, Y. L., Lin, C. F., Ho, J. Y., Huang, C. H., Chiu, C. H., Hsieh, P. W., and Horng*, J. T. (2016) An extract from Taxodium distichum targets hemagglutinin- and neuraminidase-related activities of influenza virus in vitro. Sci Rep 6, 36015

6. Chang, Y. H., Lau, K. S., Kuo, R. L., and Horng*, J. T. (2017) dsRNA Binding Domain of PKR Is Proteolytically Released by Enterovirus A71 to Facilitate Viral Replication. Front Cell Infect Microbiol 7, 284

7. Chen, S. G., Cheng, M. L., Chen, K. H., Horng, J. T., Liu, C. C., Wang, S. M., Sakurai, H., Leu, Y. L., Wang, S. D., and Ho, H. Y. (2017) Antiviral activities of Schizonepeta tenuifolia Briq. against enterovirus 71 in vitro and in vivo. Sci Rep 7, 935

8. Jheng, J. R., Lau, K. S., Lan, Y. W., and Horng, J. T. (2018) A novel role of ER stress signal transducer ATF6 in regulating enterovirus A71 viral protein stability. Journal of biomedical science 25, 9

9. Huang, C. G., Lee, L. A., Wu, Y. C., Hsiao, M. J., Horng, J. T., Kuo, R. L., Huang, C. H., Lin, Y. C., Tsao, K. C., Chen, M. C., Chen, T. C., and Shih, S. R. (2018) A pilot study on primary cultures of human respiratory tract epithelial cells to predict patients' responses to H7N9 infection. Oncotarget 9, 14492-14508

10. Sethy, B., Hsieh, C. F., Yeh, C., Horng, J. T., and Hsieh, P. W. (2018) Design, synthesis & structure-activity relationships of a new class of antihuman enterovirus D68 & A71 agents. Future Med Chem 10, 1333-1347

11. Tang, W. F., Huang, R. T., Chien, K. Y., Tang, P., and Horng*, J. T. (2018) Large-Scale Proteomic Identification of Targets of Cellular miR-197 Downregulated by Enterovirus A71. Journal of proteome research

12. Sethy, B., Hsieh, C. F., Lin, T. J., Hu, P. Y., Chen, Y. L., Lin, C. Y., Tseng, S. N., Horng*, J. T., and Hsieh*, P. W. (2019) Design, Synthesis, and Biological Evaluation of Itaconic Acid Derivatives as Potential Anti-Influenza Agents. Journal of medicinal chemistry 62, 2390-2403

13. Hsieh, C.F., Jheng, J.R., Lin, G.H., Chen, Y.L., Ho, J.Y., Liu, C.J., Hsu, K.Y., Chen, Y.S., Chan, Y.F., Yu, H.M., Hsieh, P.W., Chern, J.H., Horng*, J.T., 2020. Rosmarinic acid exhibits broad anti-enterovirus A71 activity by inhibiting the interaction between the five-fold axis of capsid VP1 and cognate sulfated receptors. Emerg Microbes Infect 9, 1194-1205.

执行中的计画:

Ongoing Research Support

MOST 109-2320-B-182 -026 -MY3      Horng (PI)      2020/08/01  ~   2023/07/31

衣康酸衍生物抑制流感病毒核酸蛋白出核的分子机转研究

MOST 109-2634-F-182-001     Horng (co-PI)         2021/03/01  ~  2022/02/28

高教深根与特色中心计画The Featured Areas Research Center Program within the framework of the Higher Education  Sprout Project by the Ministry of Education (MOE) in Taiwan and the Ministry of Science and Technology.

MOST 109-2327-B-182 -003 -       Horng (PI)         2020/07/01  ~   2021/05/31

以新冠病毒RNA聚合酶為标的进行药物开发