91猫先生

长庚大学學術能量集萃

研究方向及研究室特色

蛋白质体学研究是指全面性的探讨蛋白质表现程度的学门。在生物医学领域中，通常着重於找出两个样品之间相对差异表现的蛋白。由於分析技术的快速进步，使得蛋白质体分析的覆盖率，已逐渐赶上基因体学研究。其中最主要的两项技术為质谱学及胜肽分离技术。本实验室主要的研究兴趣就是针对这两项技术进行研发及改进，以提升蛋白质体数据的广泛度、灵敏度、可信度及自动化程度。因此本实验室针对以下叁项蛋白体分析技术开发改进的方法:

一、建立高深度蛋白体及磷酸化蛋白体之分析技术

    建立多种奈升(nano liter)级多维液相分离系统，使一般性的整体蛋白体及磷酸化蛋白体分析深度，达到高於10000个蛋白及30000条磷酸化胜肽。

二、建立同步侦测数十个标的蛋白的灵敏技术

    结合化学修饰及层析分离技术，移除不适於定量之胜肽族群，以容许提高样品进样量，搭配多重反应监测质谱技术，同时进行数十个蛋白之定量分析。&苍产蝉辫;&苍产蝉辫;&苍产蝉辫;&苍产蝉辫; 

叁、建立分析超微量样品的分析技术

    建立超低流速的液相层析系统，提高样品进入质谱仪时之浓度，以提高侦测之灵敏度。此技术将运用於单细胞蛋白体之分析。

最近五年所发表论文

1. Chang PK, Chien KY, Chen JC.Dopamine transporter is downregulated and its association with chaperone protein Hsc70 is enhanced by activation of dopamine D3 receptor. Brain Res Bull. 2020 Dec;165:263-271
2. Chakraborty A, Lin WC, Lin YT, Huang KJ, Wang PY, Chang IY, Wang HI, Ma KT, Wang CY, Huang XR, Lee YH, Chen BC, Hsieh YJ, Chien KY, Lin TY, Liu JL, Sung LY, Yu JS, Chang YS, Pai LM. SNAP29 mediates the assembly of histidine-induced CTP synthase filaments in proximity to the cytokeratin network. J Cell Sci. 2020 May 11;133(9):jcs240200. doi: 10.1242/jcs.240200.
3. Hsiao TF, Wang CL, Wu YC, Feng HP, Chiu YC, Lin HY, Liu KJ, Chang GC, Chien KY, Yu JS, Yu CJ. Integrative omics analysis reveals soluble cadherin-3 as a survival predictor and an early monitoring marker of EGFR tyrosine kinase inhibitor therapy in lung cancer. Clin Cancer Res. 2020 Jul 1;26(13):3220-3229. doi: 10.1158/1078-0432.CCR-19-3972.
4. Cheng ML, Chien KY, Lai CH, Li GJ, Lin JF, Ho HY. Metabolic Reprogramming of Host Cells in Response to Enteroviral Infection. Cells. 2020 Feb 18;9(2). pii: E473. doi: 10.3390/cells9020473.
5. Hsiao YC, Lin SY, Chien KY, Chen SF, Wu CC, Chang YT, Chi LM, , Chiang WF, Chien CY, Chang KP, Chang YS, Yu JS. An immuno-MALDI mass spectrometry assay for the oral cancer biomarker, matrix metalloproteinase-1, in dried saliva spot samples. Anal Chim Acta. 2020 Mar 1;1100:118-130.
6. Chi LM, Hsiao YC, Chien KY, Chen SF, Chuang YN, Lin SY, Wang WS, Chang IY, Yang C, , Chiang WF, Chien CY, Chang YS, Chang KP, Yu JS. Assessment of candidate biomarkers in paired saliva and plasma samples from oral cancer patients by targeted mass spectrometry. J Proteomics. 2020 Jan 16;211:103571.
7. Tang WF, Huang RT, Chien KY, Tang P, Horng JT. A large-scale proteomic identification of targets of cellular miR-197 downregulated by enterovirus A71. J Proteome Res. 2019 Jan 4; 18(1):449-460
8. Lin WC, Chakraborty A, Huang SC, Wang PY, Hsieh YJ, Chien KY, Lee YH, Chang CC, Tang HY, Lin YT, Tung CS, Luo JD, Chen TW, Lin TY, Cheng ML, Chen YT, Yeh CT, Liu JL, Sung LY, Shiao MS, Yu JS, Chang YS, Pai LM. Histidine-Dependent Protein Methylation Is Required for Compartmentalization of CTP Synthase. Cell Rep. 2018 Sep 4;24(10):2733-2745.e7. doi: 10.1016/j.celrep.2018.08.007.
9. Huang TY, Chi LM, Chien KY. Size-exclusion chromatography using reverse-phase columns for protein separation. J Chromatogr A. 2018 Oct 12;1571:201-212. doi: 10.1016/j.chroma.2018.08.020. Epub 2018 Aug 10.
10. He JP, Hou PP, Chen QT, Wang WJ, Sun XY, Yang PB, Li YP, Yao LM, Li X, Jiang XD, Chien KY, Zhang ZM, Wu QW, Cowin AJ, Wu Q, Chen HZ. Flightless-I Blocks p62-Mediated Recognition of LC3 to Impede Selective Autophagy and Promote Breast Cancer Progression. Cancer Res. 2018 Sep 1;78(17):4853-4864. doi: 10.1158/0008-5472.CAN-17-3835. Epub 2018 Jun 13.
11. Chuang CF, King CE, Ho BW, Chien KY, Chang YC. Unbiased Proteomic Study of the Axons of Cultured Rat Cortical Neurons. J Proteome Res. 2018 May 4;17(5):1953-1966. doi: 10.1021/acs.jproteome.8b00069. Epub 2018 Apr 19.
12. Li XX, Wang ZJ, Zheng Y, Guan YF, Yang PB, Chen X, Peng C, He JP, Ai YL, Wu SF, Chien KY, Wu Q, Chen HZ. Nuclear Receptor Nur77 Facilitates Melanoma Cell Survival under Metabolic Stress by Protecting Fatty Acid Oxidation. Mol Cell. 2018 Feb 1;69(3):480-492.e7.
13. Hsiao YC, , Chen YT, Chi LM, Chien KY, Chiang WF, Chang YT, Chen SF, Wang WS, Chuang YN, Lin SY, Chien CY, Chang KP, Chang YS, Yu JS. Variability Assessment of 90 Salivary Proteins in Intraday and Interday Samples from Healthy Donors by Multiple Reaction Monitoring-Mass Spectrometry. Proteomics Clin Appl. 2018 Mar;12(2). doi: 10.1002/prca.201700039
14. Chen HY, Chang JT, Chien KY, Lee YS, You GR, Cheng AJ. The Endogenous GRP78 Interactome in Human Head and Neck Cancers: A Deterministic Role of Cell Surface GRP Cancer Stemness. Sci Rep. 2018 Jan 11;8(1):536.
15. Tsai HJ, Chien KY, Liao HR, Shih MS, Lin YC, Chang YW, Cheng JC, Tseng CP. Functional links between Disabled-2 Ser723 phosphorylation and thrombin signaling in human platelets. J Thromb Haemost. 2017 Oct;15(10):2029-2044.
16. Chen TW, Gan RC, Fang YK, Chien KY, Liao WC, Chen CC, Wu TH, Chang IY, Yang C, Huang PJ, Yeh YM, Chiu CH, Huang TW, Tang P. FunctionAnnotator, a versatile and efficient web tool for non-model organism annotation. Sci Rep. 2017 Sep 5;7(1):10430.
17. Hsiao YC, Chi LM, Chien KY, Chiang WF, Chen SF, Chuang YN, Lin SY, Wu CC, Chang YT, , Chen YT, Chia SL, Chien CY, Chang KP, Chang YS, Yu JS. Development of a Multiplexed Assay for Oral Cancer Candidate Biomarkers Using Peptide Immunoaffinity Enrichment and Targeted Mass Spectrometry. Mol Cell Proteomics. 2017 Oct;16(10):1829-1849.
18. Hsieh YJ, Chien KY, Yang IF, Lee IN, Wu CC, Huang TY, Yu JS. Oxidation of protein-bound methionine in Photofrin-photodynamic therapy-treated human tumor cells explored by methionine-containing peptide enrichment and quantitative proteomics approach. Sci Rep. 2017 May 2;7(1):1370.
19. Bian XL, Chen HZ, Yang PB, Li YP, Zhang FN, Zhang JY, Wang WJ, Zhao WX, Zhang S, Chen QT, Zheng Y, Sun XY, Wang XM, Chien KY, Wu Q. Nur77 suppresses hepatocellular carcinoma via switching glucose metabolism toward gluconeogenesis through attenuating phosphoenolpyruvate carboxykinase sumoylation. Nat Commun. 2017 Feb 27;8:14420.
20. Lin YH, Zhen YY, Chien KY, Lee IC, Lin WC, Chen MY, Pai LM. LIMCH1 regulates nonmuscle myosin-II activity and suppresses cell migration. Mol Biol Cell. 2017 Apr 15;28(8):1054-1065.
21. Lin YT, Chien KY, Wu CC, Chang WY, , Chen MC, Yeh CT, Yu JS. Super-SILAC mix coupled with SIM/AIMS assays for targeted verification of phosphopeptides discovered in a large-scale phosphoproteome analysis of hepatocellular carcinoma. J Proteomics. 2017 Mar 22;157:40-51.
22. Kung YA, Hung CT, Chien KY, Shih SR. Control of the negative IRES trans-acting factor KHSRP by ubiquitination. Nucleic Acids Res. 2017 Jan 9;45(1):271-287.